Antibody:	Ab79
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to beta-galactosidase

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from head mesoderm	Present		2D	Mesodermal expression was detected along the entire length of the head process and notochord.
TS15: notochord	Present		2D	Mesodermal expression was detected along the entire length of the head process and notochord.
TS15: future brain	Present	Regionally restricted	2D	Expression was restricted to the ventral midline in most of the brain, with the exception of the rostral diencephalon where expression skirts the presumptive hypothalamic region, the paramedian plate.
TS15: future spinal cord	Present	Regionally restricted	2D	Ventral midline expression in the spinal cord extended caudally to the position of the 4th and 5th most recently formed somites.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present	Regionally restricted		Expression becomes restricted to the notochord caudal to the forelimb buds at E11.5.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted		Thus, there is a continuous ventral strip of expression at, or close to, the ventral midline of the central nervous system (CNS) extending from the rostral limit of the CNS in the forebrain to caudal regions of the spinal cord. This expression persists in the mouse until at least E15.5.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted		There was a dorsal expansion of expression at the zona limitans intrathalamica at E10.5.