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Gene Expression Data
Assay Details
Assay
Reference: J:200651 Garcia-Lopez J, et al., Reprogramming of microRNAs by adenosine-to-inosine editing and the selective elimination of edited microRNA precursors in mouse oocytes and preimplantation embryos. Nucleic Acids Res. 2013 May 1;41(10):5483-93
Assay type: RT-PCR
MGI Accession ID: MGI:6201293
Gene symbol: Adar
Gene name: adenosine deaminase, RNA-specific
Probe: Adar1p110-F, Adar1p110-R
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression is shown normalized to Ppia and H2afz expression.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Oocyte P adult TS28: oocyte Strong (a) 0.5 µg; total RNA Not Specified Female
Zygote E0.5 (b) TS1: 1-cell stage conceptus Strong (a) 0.5 µg; total RNA Not Specified Not Specified
2-cell embryo E1.0 (b) TS2: 2-cell stage conceptus Present 0.5 µg; total RNA Not Specified Not Specified
4-cell embryo E2.0 (b) TS3: 4-cell stage conceptus Trace 0.5 µg; total RNA Not Specified Not Specified
8-cell embryo E2.5 (b) TS3: 8-cell stage conceptus Present 0.5 µg; total RNA Not Specified Not Specified
Morula E3.0 (b) TS3: morula-stage conceptus Trace 0.5 µg; total RNA Not Specified Not Specified
Blastocyst E3.5 (b) TS4: blastocyst-stage conceptus Absent (c) 0.5 µg; total RNA Not Specified Not Specified
Testis P TS28: testis Present 0.5 µg; total RNA Not Specified Male
Brain P TS28: brain Present 0.5 µg; total RNA Not Specified Not Specified
Notes:
(a) There were about 2x more transcripts than in brain and 10x more than in testis.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) Absent assigned by curator.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory