Dnmt1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:135338 Cirio MC, et al., Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints. BMC Dev Biol. 2008;8:9
Assay type:	Western blot
MGI Accession ID:	MGI:6201240
Gene symbol:	Dnmt1
Gene name:	DNA methyltransferase 1

Antibody:	UPT82
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Antibody recognizes only the Dnmt1s proteins.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex	Note
MII	P21-35	TS28: secondary oocyte	Present	Not Specified		Female	metaphase II stage oocyte
1	E0.5 (a)	TS1: 1-cell stage embryo	Present (b)	Not Specified		Not Specified
2	E1.5	TS2: 2-cell stage embryo	Present (c)	Not Specified		Not Specified
4	E2.0 (a)	TS3: 4-cell stage embryo	Present (c)	Not Specified		Not Specified
8	E2.5 (a)	TS3: 8-cell stage embryo	Present	Not Specified		Not Specified
Bl	E3.5	TS4: blastocyst	Present (d)	Not Specified		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Similar expression compared to that seen in wild-type MII oocytes.
(c) Lower expression compared to that seen in wild-type MII oocytes.
(d) Highest expression, 7 times the MII-oocyte concentration and approximately 18 times the 4-cell concentration..

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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