Matn4 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:69493 Klatt AR, et al., Molecular structure, processing, and tissue distribution of matrilin-4. J Biol Chem. 2001 May 18;276(20):17267-75
Assay type:	Western blot
MGI Accession ID:	MGI:6201227
Gene symbol:	Matn4
Gene name:	matrilin 4

Antibody:	Anti-Matrilin-4
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Lanes represent sequential extractions of the protein sample. Differential solubility of the individual oligomeric forms was noted.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
TBS	P newborn	TS27: brain	Ambiguous (a)	Not Specified		Not Specified
EDTA	P newborn	TS27: brain	Present (b)	Not Specified		Not Specified
Urea	P newborn	TS27: brain	Present (c)	Not Specified		Not Specified
GuHCl	P newborn	TS27: brain	Present (d)	Not Specified		Not Specified

Notes:
(a) All forms could not be solubilized in TBS alone.
(b) All forms could be solubilized in EDTA-containing buffer.
(c) Denaturation with urea released large additional amounts of the trimeric form and the 170kDa form.
(d) Further denaturation with 4M GdnHCl released only the trimeric form of the protein.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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