Antibody:	Anti-GnRH/LHRH (20075)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Three antibodies were described SW1 MGI:3043770, LR5 MGI:5467573, and LHRH-A MGI:5619842; the one used in this assay was not specified. The publication noted that a similar staining pattern was obtained with all antibodies.

Structure	Level	Pattern	Image	Note
TS19: dental placode	Absent			No expression was detected in incisors at E11.5.

Structure	Level	Pattern	Image	Note
TS21: lower jaw incisor epithelium	Present	Regionally restricted	1F	Expression was localized in cells in the anterior aspect of the dental epithelium.
TS21: oral epithelium	Present	Regionally restricted	1F	Expression was detected in cells in oral epithelium adjacent to the developing tooth bud.

Structure	Level	Pattern	Image	Note
TS28: upper jaw incisor	Present	Regionally restricted	2A	Expression was detected in cells on the labial side but not the lingual aspect. Staining on the labial side was weak in the posterior aspect, but robust in the more incisal (distal) aspect. Staining was absent at the tip of the incisors and in the most proximal (apical) aspect of incisors.
TS28: upper jaw incisor epithelium	Present	Regionally restricted	2A	Expression was detected in cells in dental epithelial-derived structures, including outer dental epithelium and enamel-secreting ameloblasts.
TS28: stellate reticulum	Present		2A
TS28: stratum intermedium	Present		2A
TS28: dental sac	Absent		2A
TS28: upper jaw incisor dental papilla	Absent		2A
TS28: upper jaw incisor mesenchyme	Absent		2A	Mesenchyme-derived structures that include dental papilla, dental follicle, and dentin-secreting odontoblast cells did not show immunoreactivity.

Structure	Level	Pattern	Image	Note
TS28: upper jaw molar	Absent		2C

Structure	Level	Pattern	Image	Note
TS27: lower jaw incisor	Present	Regionally restricted	4D , 4A	Expression was detected in ameloblasts.
TS27: stellate reticulum	Present		4A , 4D
TS27: stratum intermedium	Present		4A , 4D

Structure	Level	Pattern	Image	Note
TS27: lower jaw incisor	Absent		4C , 4F

Structure	Level	Pattern	Image	Note
TS27: lower jaw incisor	Present	Regionally restricted	4E	Expression was detected in ameloblasts.
TS27: stellate reticulum	Present		4E
TS27: stratum intermedium	Present		4E