Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:26506
Vielkind U, et al., Dynamics of Merkel cell patterns in developing hair follicles in the dorsal skin of mice, demonstrated by a monoclonal antibody to mouse keratin 8. Acta Anat (Basel). 1995;152(2):93-109
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6199445
|
|
Gene symbol:
|
Ncam1
|
|
Gene name:
|
neural cell adhesion molecule 1
|
|
Antibody:
|
anti-NCAM [H28.123]
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
Dorsolateral skin was examined in all cases. Tabby mutants were either Ta/Ta or Ta/Y.
|
|
|
Results
|
Specimen
1B:
embryonic day 13.0
(more  )
Note: Prefollicle stage.
Specimen
1B:
(close  )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 13.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Prefollicle stage.
|
Specimen
2D:
embryonic day 14.0
(more )
Note: Tylotrich follicle stage 1 (placode).
Specimen
2D:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 14.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 1 (placode).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: dermis |
Present |
Regionally restricted |
2D
|
Expression is in nerve endings in the upper dermis and in the layer of differentiating cutaneous muscle cells deep in the dermis.
|
Specimen
3E:
embryonic day 15.0
(more )
Note: Tylotrich follicle stage 2 (hair plug).
Specimen
3E:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 15.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 2 (hair plug).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dermis |
Present |
Regionally restricted |
3E
|
Expression is in nerve endings in the upper dermis and is weak in mesenchymal cells below the epidermis.
|
| TS23: hair epidermal placode |
Present |
|
3E
|
|
Specimen
4F:
embryonic day 16.0
(more )
Note: Tylotrich follicle stage 3a (early dermal papillae).
Specimen
4F:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 16.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 3a (early dermal papillae).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: dermis |
Present |
Regionally restricted |
4F
|
Expression is in nerve endings in the upper dermis.
|
| TS24: hair epidermal placode |
Present |
Regionally restricted |
4F
|
Expression is in the caudal part.
|
| TS24: epidermis |
Present |
Single cells |
4F
|
Expression is in a few cells adjacent to the tylotrich follicle.
|
Specimen
5C:
embryonic day 17.0
(more )
Note: Tylotrich follicle stage 3b (developing dermal papillae).
Specimen
5C:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 3b (developing dermal papillae).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hair follicle |
Present |
Regionally restricted |
5C
|
Expression is in basal cells in the upper caudal part of the tylotrich follicle.
|
| TS25: dermis |
Present |
Regionally restricted |
5C
|
Expression is in mesenchymal cells in the upper dermis particularly surrounding the follicles and those forming the dermal papillae.
|
| TS25: epidermis |
Present |
Single cells |
5C
|
Expression is in a few cells adjacent to the tylotrich follicle.
|
| TS25: hair follicle dermal papilla |
Present |
|
5C
|
|
Specimen
6D:
embryonic day 18.0
(more )
Note: Tylotrich follicle stage 3c (completed dermal papillae).
Specimen
6D:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 18.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 3c (completed dermal papillae).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
6D
|
Expression is in basal cells in the upper caudal part of the tylotrich and awl follicles.
|
| TS26: dermis |
Present |
Regionally restricted |
6D
|
Expression is in mesenchymal cells in the upper dermis, in dermal papillae, and in the flattened mesenchymal sheath around the lower part of each follcile.
|
| TS26: hair follicle dermal papilla |
Present |
|
6D
|
|
Specimen
not shown E19:
embryonic day 19.0
(more )
Note: Tylotrich follicle stage 4 (early inner root sheath cone).
Specimen
not shown E19:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J/JGbms
|
|
Age:
|
embryonic day 19.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 4 (early inner root sheath cone).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dermis |
Present |
Regionally restricted |
|
Expression is in mesenchymal cells in the upper dermis, in dermal papillae, and in the flattened mesenchymal sheath around the lower part of each follcile.
|
| TS26: hair follicle |
Present |
Regionally restricted |
|
Expression is in basal cells in the upper caudal part of the tylotrich and awl follicles.
|
| TS26: hair follicle dermal papilla |
Present |
|
|
|
Specimen
9B:
embryonic day 14.0; EdaTa/ EdaTa
(more )
Specimen
9B:
(close )
|
Genetic Background:
|
B6.Cg-Aw-J EdaTa/JGbms
|
|
Mutant Allele(s):
|
EdaTa/EdaTa
|
|
Age:
|
embryonic day 14.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: dermis |
Present |
Regionally restricted |
9B
|
Expression is in 2-3 layers of cutaneous myoblasts, in 1-2 layers of mesenchymal cells immediately below the epidermis, and in axons and nerve endings.
|
Specimen
not shown Tabby E16:
embryonic day 16.0; EdaTa/ EdaTa
(more )
Specimen
not shown Tabby E16:
(close )
|
Genetic Background:
|
B6.Cg-Aw-J EdaTa/JGbms
|
|
Mutant Allele(s):
|
EdaTa/EdaTa
|
|
Age:
|
embryonic day 16.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: dermis |
Present |
Regionally restricted |
10B
|
Expression is in a few nerve endings.
|
Specimen
not shown Tabby E17:
embryonic day 17.0; EdaTa/ EdaTa
(more )
Note: Awl follicle stage 2.
Specimen
not shown Tabby E17:
(close )
|
Genetic Background:
|
B6.Cg-Aw-J EdaTa/JGbms
|
|
Mutant Allele(s):
|
EdaTa/EdaTa
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Awl follicle stage 2.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: dermis |
Present |
Regionally restricted |
11B
|
Expression is in a few nerve endings, in mesenchymal cells immediately below the epidermis, and in cells surrounding the awl hair follciles.
|
| TS25: hair epidermal placode |
Trace |
|
11B
|
Expression was mostly negative.
|
Specimen
not shown Tabby E18:
embryonic day 18.0; EdaTa/ EdaTa
(more )
Note: Awl follicle stage 3a.
Specimen
not shown Tabby E18:
(close )
|
Genetic Background:
|
B6.Cg-Aw-J EdaTa/JGbms
|
|
Mutant Allele(s):
|
EdaTa/EdaTa
|
|
Age:
|
embryonic day 18.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Awl follicle stage 3a.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
12B
|
Expression is in the upper part of the awl follicle.
|
| TS26: dermis |
Present |
Regionally restricted |
12B
|
Expression is in the mesenchymal sheath around the awl follicles, the upper dermis between the follicles, and in occasional axons.
|
Specimen
not shown Tabby E19:
embryonic day 19.0; EdaTa/ EdaTa
(more )
Note: Awl follicle stage 3b.
Specimen
not shown Tabby E19:
(close )
|
Genetic Background:
|
B6.Cg-Aw-J EdaTa/JGbms
|
|
Mutant Allele(s):
|
EdaTa/EdaTa
|
|
Age:
|
embryonic day 19.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Awl follicle stage 3b.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
|
Expression is in the upper part of the awl follicle.
|
| TS26: dermis |
Present |
Regionally restricted |
|
Expression is in the mesenchymal sheath around the awl follicles, the upper dermis between the follicles, and in occasional axons.
|
Specimen
not shown downy E15.0:
embryonic day 15.0; dny/ dny
(more )
Note: Tylotrich follicle stage 2.
Specimen
not shown downy E15.0:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J dny/Gbms
|
|
Mutant Allele(s):
|
dny/dny
|
|
Age:
|
embryonic day 15.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 2.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dermis |
Present |
|
|
Expression in this mutant is the same as in wild-type.
|
| TS23: hair follicle |
Present |
|
|
Expression in this mutant is the same as in wild-type.
|
Specimen
not shown downy E17.0:
embryonic day 17.0; dny/ dny
(more )
Note: Tylotrich follicle stage 3b.
Specimen
not shown downy E17.0:
(close )
|
Genetic Background:
|
C57BL/6J-Aw-J dny/Gbms
|
|
Mutant Allele(s):
|
dny/dny
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Tylotrich follicle stage 3b.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: dermis |
Present |
|
|
Expression in this mutant is the same as in wild-type.
|
| TS25: hair follicle |
Present |
|
|
Expression in this mutant is the same as in wild-type.
|
|
Analysis Tools