Antibody:	anti-NCAM
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		1c	Expression outlined the neuroepithelial cells.
TS17: somite	Present	Regionally restricted	1c	Expression was concentrated along the remnants of the somite cavity.
TS17: dorsal root ganglion	Weak		1c
TS17: notochord	Present		1c
TS17: segmental spinal nerve	Strong		1c	Highly expressed in the ventral root fibers.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak	Regionally restricted	1d	Lightly labeled putative neural crest cells can be seen emigrating from the dorsal spinal cord.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		2a

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2b	Clumps of delicately outlined cells were seen at the lateral ends of the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: somite	Present		2c	The somite is irregular and not as elongated as in nomal embryos of comparable age.
TS17: dorsal root ganglion	Present		2c	Less evident than in the neuroepithelium.
TS17: neural tube	Present		2c	Expression outlined the neuroepithelial cells.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		2d
TS17: somite	Present		2d
TS17: segmental spinal nerve	Present	Regionally restricted	2d	Expressed in the ventral root fibers.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Strong	Regionally restricted	3a	Expressed in the ventral horn.
TS19: segmental spinal nerve	Strong	Regionally restricted	3a	Expressed in the ventral roots and dorsal root bifurcation zone.
TS19: neural tube marginal layer	Strong	Regionally restricted	3a	Expressed at the ventral midline, representing commissurafibers crossing the midline.
TS19: dorsal root ganglion	Present		3a
TS19: somite	Present		3a

Structure	Level	Pattern	Image	Note
TS20: segmental spinal nerve	Strong	Regionally restricted	3b	Expressed in dorsal root fibers passing dorso-medially from the DRG to the spinal cord.
TS20: spinal cord	Present	Regionally restricted	3b	Expressed in the dorsolateral neural tube and the marginal layer.
TS20: dorsal root ganglion	Present		3b

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	3c	Expressed at the ventral midline, representing commissurafibers crossing the midline.
TS20: notochord	Weak		3c
TS20: trunk mesenchyme	Strong	Regionally restricted	3c	Expressed in the mesenchymal cells that have condensed in sworls around the notochord.

Structure	Level	Pattern	Image	Note
TS20: notochord	Weak		3d
TS20: trunk mesenchyme	Strong	Regionally restricted	3d	Expressed in the mesenchymal cells that have condensed in sworls around the notochord.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Strong	Regionally restricted	4a	Expressed in the ventral horn cells.
TS19: segmental spinal nerve	Strong	Regionally restricted	4a	Expressed in the ventral roots.
TS19: dorsal root ganglion	Present		4a
TS19: somite	Present		4a

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	4b	Expressed in the marginal layer and the ventral horn.
TS20: segmental spinal nerve	Strong	Regionally restricted	4b	Expressed in the ventral root fibers passing dorsolateraly from the DRG to the spinal cord.
TS20: dorsal root ganglion	Present		4b

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4c	Expressed in a narrow marginal layer at the ventral midline, exhibiting labeled ventral commissural fibers.

Structure	Level	Pattern	Image	Note
TS20: notochord	Weak		4d
TS20: trunk mesenchyme	Present	Regionally restricted	4d	Expressed in the mesenchymal cells that have condensed in sworls around the notochord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord roof plate	Weak		5	Expressed in a few orderly layers of wedge-shaped cells.

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Present		6a	Expressed in neuroepithelial cells in a hook-like 'roof plate'.

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Present		6b	Expressed in neuroepithelial cells in a hook-like 'roof plate'.

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Present		6c	Expressed in neuroepithelial cells in a hook-like 'roof plate'.

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Present		6d	Expressed in neuroepithelial cells in a hook-like 'roof plate'.

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Present		6e	The 'roof plate' contains a nodule of rounded well-labeled cells projecting from the 'luminal' surface of the neuroepitheliium into the amniotic cavify.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Uniform

Structure	Level	Pattern	Image	Note
TS20: spinal cord roof plate	Present			Expressed in neuroepithelial cells in a hook-like 'roof plate'.