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Gene Expression Data
Assay Details
Assay
Reference: J:234164 Geffers I, et al., Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo. J Cell Biol. 2007 Jul 30;178(3):465-76
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6198637
Gene symbol: Notch1
Gene name: notch 1
Antibody: anti-cleaved Notch1 (Val1744) antibody (anti-NICD) (#2421)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 3A: embryonic day 10.5 (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Present Regionally restricted 3A In wild type embryos, Notch1 is activated in a sharp band in the anterior presomitic mesoderm, and posterior in variable patterns that reflect cyclic Notch activity.

Specimen 3B: embryonic day 10.5 (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Present Regionally restricted 3B In wild type embryos, Notch1 is activated in a sharp band in the anterior presomitic mesoderm, and posterior in variable patterns that reflect cyclic Notch activity.

Specimen 3C: embryonic day 10.5 (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Present Regionally restricted 3C In wild type embryos, Notch1 is activated in a sharp band in the anterior presomitic mesoderm, and posterior in variable patterns that reflect cyclic Notch activity.

Specimen 3D: embryonic day 10.5; Dll1tm1Gos/Dll1tm1Gos (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Absent 3D

Specimen 3E: embryonic day 10.5; Dll3pu/Dll3pu (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Present Regionally restricted 3E Expression was detected in a fuzzy stripe in the anterior presomitic mesoderm and at the posterior end, but not throughout the presomitic mesoderm, and levels appeared reduced rather than increased.

Specimen 3F: embryonic day 10.5; Dll3pu/Dll3pu (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Present Regionally restricted 3F Expression was detected in a fuzzy stripe in the anterior presomitic mesoderm and at the posterior end, but not throughout the presomitic mesoderm, and levels appeared reduced rather than increased.

Specimen 3G: embryonic day 10.5; Dll3pu/Dll3pu (more )
Note: Fixation: Methanol/DMSO/30% H2O2.
Structure Level Pattern Image Note
TS17: tail unsegmented mesenchyme Present Regionally restricted 3G Expression was detected in a fuzzy stripe in the anterior presomitic mesoderm and at the posterior end, but not throughout the presomitic mesoderm, and levels appeared reduced rather than increased.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory