Efhd2 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:223142 Purohit P, et al., The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. PLoS One. 2014;9(8):e103976
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6198026
Gene symbol:	Efhd2
Gene name:	EF hand domain containing 2

Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative was 0.2% glutaraldehyde and a post fixative with 4% paraformaldehyde.

Results

Specimen 1C female dorsal: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present		1C +/- dorsal female

Specimen 1C female ventral: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present		1C +/- ventral female

Specimen 1C male dorsal: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present		1C +/- dorsal male

Specimen 1C male ventral: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present		1C +/- ventral male

Specimen 1D +/- anterior: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2⁺ (more

)

Structure	Level	Image
TS28: cortical layer IV	Present	1D +/- anterior
TS28: cortical layer V	Present	1D +/- anterior
TS28: cortical layer VI	Present	1D +/- anterior
TS28: dentate gyrus	Present	1D +/- anterior
TS28: hippocampus CA1	Present	1D +/- anterior
TS28: hippocampus CA2	Present	1D +/- anterior
TS28: main olfactory bulb	Present	1D +/- anterior
TS28: thalamus	Present	1D +/- anterior

Specimen 1D -/- anterior: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2^{tm1(KOMP)Vlcg} (more

)

Structure	Level	Image
TS28: cortical layer IV	Strong	1D -/- anterior
TS28: cortical layer V	Strong	1D -/- anterior
TS28: cortical layer VI	Strong	1D -/- anterior
TS28: dentate gyrus	Strong	1D -/- anterior
TS28: hippocampus CA1	Strong	1D -/- anterior
TS28: hippocampus CA2	Strong	1D -/- anterior
TS28: main olfactory bulb	Strong	1D -/- anterior
TS28: thalamus	Strong	1D -/- anterior

Specimen 1D +/- posterior: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2⁺ (more

)

Structure	Level	Image
TS28: cortical layer IV	Present	1D +/- posterior
TS28: cortical layer V	Present	1D +/- posterior
TS28: cortical layer VI	Present	1D +/- posterior
TS28: dentate gyrus	Present	1D +/- posterior
TS28: hippocampus CA1	Present	1D +/- posterior
TS28: hippocampus CA2	Present	1D +/- posterior
TS28: main olfactory bulb	Present	1D +/- posterior
TS28: thalamus	Present	1D +/- posterior

Specimen 1D -/- posterior: postnatal adult; Efhd2^{tm1(KOMP)Vlcg}/Efhd2^{tm1(KOMP)Vlcg} (more

)

Structure	Level	Image
TS28: cortical layer IV	Strong	1D -/- posterior
TS28: cortical layer V	Strong	1D -/- posterior
TS28: cortical layer VI	Strong	1D -/- posterior
TS28: dentate gyrus	Strong	1D -/- posterior
TS28: hippocampus CA1	Strong	1D -/- posterior
TS28: hippocampus CA2	Strong	1D -/- posterior
TS28: main olfactory bulb	Strong	1D -/- posterior
TS28: thalamus	Strong	1D -/- posterior

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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