Gene Expression Data
Assay Details
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Assay
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Reference:
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J:145734
Daubas P, et al., The regulatory mechanisms that underlie inappropriate transcription of the myogenic determination gene Myf5 in the central nervous system. Dev Biol. 2009 Mar 1;327(1):71-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6198006
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5D:
embryonic day 11.5
(more  )
Specimen
5D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
5E:
embryonic day 11.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: prosomere 1 |
Present |
Regionally restricted |
5E
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Expression in neuroepithelium of prosomere p1 region of brain.
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Specimen
wild type not shown:
embryonic day 11.5
(more )
Specimen
wild type not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: prosomere |
Present |
Regionally restricted |
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| TS19: limb bud |
Present |
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Expression in somites and limb buds in both wild type and mutant embryos.
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| TS19: somite |
Present |
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Expression in somites and limb buds in both wild type and mutant embryos.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pou3f1tm1Mejr/Pou3f1tm1Mejr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain |
Absent |
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5G
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No expression in neuroepithelium of mutant.
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Specimen
mutant not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pou3f1tm1Mejr/Pou3f1tm1Mejr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb bud |
Present |
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Expression in somites and limb buds in both wild type and mutant embryos.
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| TS19: somite |
Present |
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Expression in somites and limb buds in both wild type and mutant embryos.
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Analysis Tools