Gene Expression Data
Assay Details
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Assay
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Reference:
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J:145693
Griffith AV, et al., Increased thymus- and decreased parathyroid-fated organ domains in Splotch mutant embryos. Dev Biol. 2009 Mar 1;327(1):216-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6197968
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Gene symbol:
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Foxn1
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Gene name:
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forkhead box N1
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Probe:
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Foxn1 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
6A:
embryonic day 11.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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involves: C57BL * C57BL/6J
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: thymus/parathyroid primordium |
Present |
Regionally restricted |
6B
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Authors report an increase in the thymus domain in mutants compared to wild type.
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Specimen
8A:
embryonic day 12.0
(more )
Specimen
8A:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foregut gland |
Present |
Regionally restricted |
8A
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Authors report a well delineated, straight boundary between thymus- and parathyroid-fated domains in third pharygeal pouch endoderm.
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Specimen
8B:
(close )
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Genetic Background:
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involves: C57BL * C57BL/6J
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foregut gland |
Present |
Regionally restricted |
8B
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Expression domain is interspersed withGcm2-expressing domain in dorsal aspect of third pharyngeal pouch, rather than restricted to ventral aspect of primordium.
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Specimen
8E:
(close )
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Genetic Background:
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involves: C57BL * C57BL/6J
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pharynx epithelium |
Present |
Regionally restricted |
8E
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Expression domain extended well into pharyngeal endoderm exterior to the pouch.
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Analysis Tools