Gene Expression Data
Assay Details
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Assay
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Reference:
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J:249891
Giri D, et al., Novel FOXA2 mutation causes Hyperinsulinism, Hypopituitarism with Craniofacial and Endoderm-derived organ abnormalities. Hum Mol Genet. 2017 Nov 15;26(22):4315-4326
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6197917
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Gene symbol:
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Foxa2
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Gene name:
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forkhead box A2
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Probe:
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Foxa2 probe15
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 11.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2B:
embryonic day 12.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2B':
embryonic day 12.5
(more )
Specimen
2B':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2C:
embryonic day 13.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: adenohypophysis |
Present |
Regionally restricted |
2C
,
2C'
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Expression was localized to the ventral side of the anterior lobe of the developing pituitary gland with transcripts in the ventral marginal zone.
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Specimen
2D:
embryonic day 15.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: adenohypophysis |
Strong |
Regionally restricted |
2D'
,
2D
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Robust expression was detected in the ventral diencephalon, posterior lobe and anterior lobe of the pituitary gland.
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| TS23: diencephalon |
Strong |
Regionally restricted |
2D'
,
2D
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Robust expression was detected in the ventral diencephalon, posterior lobe and anterior lobe of the pituitary gland.
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| TS23: neurohypophysis |
Strong |
Regionally restricted |
2D'
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2D
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Robust expression was detected in the ventral diencephalon, posterior lobe and anterior lobe of the pituitary gland.
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Specimen
2E:
embryonic day 18.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: central nervous system |
Present |
Widespread |
2E
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| TS26: telencephalon |
Strong |
Regionally restricted |
2E
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Strong expression was detected in the lumen surrounding the lateral ventricles.
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| TS26: diencephalon |
Strong |
Regionally restricted |
2E
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Strong expression was detected in the lumen surrounding the third ventricle.
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| TS26: hypothalamus |
Strong |
Regionally restricted |
2E
,
2E'
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There was a distinct pattern in the luminal area where the hypothalamic precursors reside. Strong expression was noted in the ventral hypothalamus.
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| TS26: adenohypophysis |
Present |
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2E
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2E'
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| TS26: neurohypophysis |
Present |
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2E
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2E'
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Analysis Tools