Gene Expression Data
Assay Details
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Assay
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Reference:
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J:264474
Mya N, et al., Transcription factor Foxc1 is involved in anterior part of cranial base formation. Congenit Anom (Kyoto). 2018 Sep;58(5):158-166
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6197237
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Probe:
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Col2a1 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A:
embryonic day 12.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: head mesenchyme |
Present |
Regionally restricted |
4A
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Expression in prechondrogenic mesenchymal condensation. Thin line of expression domain - posterior tipe dilated and blurred - appears to correspond to basal portion of trabecular cartilage and anterior portion of hypophyseal cartilage. Expression in mesenchymal condensation for posterior portion of hypophyseal cartilage just posterior to epithelial stalk of Rathke's pouch.
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Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6 * CHMU/Le
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Mutant Allele(s):
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Foxc1ch/Foxc1ch
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: head mesenchyme |
Present |
Regionally restricted |
4B
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Reduced expression in posterior part of basal portion of trabecular cartilage and anterior portion of hypophyseal cartilage. Normal expression in primordium of posterior part of hypophyseal cartilage.
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Specimen
4C:
embryonic day 13.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: chondrocranium pre-cartilage condensation |
Present |
Regionally restricted |
4C
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Decreased expression between trabecular cartilage and hypophyseal cartilage. Expression domains of posterior tip of basal portion of trabecular cartilage and anteior hypophyseal artilage connected to each other.
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Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6 * CHMU/Le
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Mutant Allele(s):
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Foxc1ch/Foxc1ch
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: chondrocranium pre-cartilage condensation |
Present |
Regionally restricted |
4D
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Reduced expression in posterior part of basal portion of trabecular cartilage. No expression in anterior portion of hypophyseal cartilage.
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Specimen
4E:
embryonic day 14.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
Present |
Regionally restricted |
4E
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Decreased expression at connection between basal portion of trabecular cartilage and hypophyseal cartilage.
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Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL/6 * CHMU/Le
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Mutant Allele(s):
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Foxc1ch/Foxc1ch
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
Present |
Regionally restricted |
4F
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Reduced expression in posterior part of basal portion of trabecular cartilage and anterior portion of hypophyseal cartilage, with no connection between. Expression for posterior portion of hypophyseal cartilage posterior to epithelial stalk.
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