Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142873
Collins CA, et al., Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling. Dev Biol. 2008 Dec 1;324(1):55-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6196085
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Gene symbol:
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Crabp2
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Gene name:
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cellular retinoic acid binding protein II
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Antibody:
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CRABP2 antibody
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Detection system:
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Alexa Fluor 488
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-Krt14 (MGI:6196068) may have been used in this assay.
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Results
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Specimen
1k:
postnatal day 2
(more  )
Specimen
1k:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: sebaceous gland |
Present |
Regionally restricted |
1k
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Expression in inner root sheath of developing sebaceous glands of occasional hair follicles.
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Specimen
1m:
postnatal day 5
(more )
Specimen
1m:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Widespread |
1m
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Expression in all hair follicles.
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| TS28: sebaceous gland |
Strong |
Regionally restricted |
1m
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Relative intense expression in nuclei of upper part of sebaceous gland.
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| TS28: epidermis suprabasal layer |
Strong |
Regionally restricted |
1m
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Strong expression in occasional nuclei of suprabasal interfollicular epidermis (IFE). Lower levels of expression in cytoplasm of suprabasal IFE cells.
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| TS28: hair follicle dermal papilla |
Weak |
Regionally restricted |
1m
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Lower levels of expression in some, but not all, dermal papillae.
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Specimen
1p:
postnatal day 42
(more )
Specimen
1p:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 42
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Weak |
Regionally restricted |
1p
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Reduced intensity of expression in telogen hair follicles.
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Specimen
1q:
postnatal day 10.2
(more )
Specimen
1q:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 10.2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair inner root sheath |
Strong |
Regionally restricted |
1q
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Much increased intensity of expression in inner rooth sheath of anagen hair follicles.
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Specimen
5b:
postnatal day 293
(more )
Specimen
5b:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 293
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Weak |
Regionally restricted |
5b
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Authors report pattern of expression essentially similar to that seen in telogen skin of 42-day-old animals.
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Analysis Tools