Probe:	Dll4 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Strong	Regionally restricted	1H	Expression was strong in the developing trophoblast. Signal was specific in discrete regions of trophoblast.
TS11: ectoplacental cone	Present	Regionally restricted	1H	Expression was detected in the upper part of the ectoplacental cone. Some cells in the mid-ectoplacental cone also expressed Dll4.

Structure	Level	Pattern	Image	Note
TS20: trophectoderm	Strong			Expression was strong in the developing trophoblast. Signal was specific in discrete regions of trophoblast.

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present	Regionally restricted		Expression was detected in the upper part of the ectoplacental cone. Some cells in the mid-ectoplacental cone also expressed Dll4.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Present	Regionally restricted		Expression was detected in the junctional zone.

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Present	Regionally restricted	4D , 4B	Expression was detected in parietal trophoblast giant cells.