Probe:	Notch2 cDNA11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Strong	Regionally restricted	1C	Expression was strong in the developing trophoblast. Signal was specific in discrete regions of trophoblast.
TS11: ectoplacental cone	Present	Regionally restricted	1C	Expression was detected in the lower part of the ectoplacental cone. Some cells in the mid-ectoplacental cone also expressed Notch2.
TS11: chorion	Present	Regionally restricted	1C	Expression was detected in the upper part of the chorion.

Structure	Level	Pattern	Image	Note
TS20: trophectoderm	Strong			Expression was strong in the developing trophoblast. Signal was specific in discrete regions of trophoblast.

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present	Regionally restricted		Expression was detected in the lower part of the ectoplacental cone. Some cells in the mid-ectoplacental cone also expressed Notch2.
TS13: chorion	Present	Regionally restricted		Expression was detected in the upper part of the chorion.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Present	Regionally restricted		Expression was detected in the junctional zone.

Structure	Level	Pattern	Image	Note
TS20: trophectoderm	Present	Regionally restricted	2_Notch2	Notch2 was detected in spongiotrophoblast cells, glycogen cells.
TS20: trophoblast giant cell	Present	Regionally restricted	2_Notch2	Expression was most strongly detected in trophoblast giant cells that span the junctional zone.

Structure	Level	Pattern	Image	Note
TS11: chorion	Strong	Regionally restricted	3_E7.5 Notch2	Strongest expression was detected mainly in the upper layer of the chorion.

Structure	Level	Pattern	Image	Note
TS13: chorion	Present	Regionally restricted	3_E8.5 Notch2 Boxed Region , 3_E8.5 Notch2	Expression was detected in a layer of cells adjacent to Jag1 and Jag2 staining in the mid-chorion.

Structure	Level	Pattern	Image	Note
TS15: labyrinthine zone	Present	Regionally restricted	3_E9.5 Notch2 , 3_E9.5 Notch2 Boxed Region	Expression was detected in the outermost thick cell layer of early labyrinth branches, and in stromal cells of the labyrinth.
TS15: trophoblast giant cell	Present	Regionally restricted	3_E9.5 Notch2 Boxed Region , 3_E9.5 Notch2	Expression was detected in cells lining maternal blood sinusoids.

Structure	Level	Pattern	Image	Note
TS17: trophoblast giant cell	Strong		3_E10.5 Notch2	Expression was detected in sinusoidal trophoblast giant cells.
TS17: labyrinthine zone	Present	Regionally restricted	3_E10.5 Notch2	Expression was detected in stromal cells.

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Strong		3_E12.5 Notch2 , 3_E12.5 Notch2 Boxed Region	Expression was detected in sinusoidal trophoblast giant cells.
TS20: labyrinthine zone	Present	Regionally restricted	3_E12.5 Notch2 , 3_E12.5 Notch2 Boxed Region	Expression was detected in stromal cells.

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Present	Regionally restricted	4E , 4A	Expression was detected in the parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	4E , 4A	Notch2 positive decidual stromal cells were detected around the implantation site.

Structure	Level	Pattern	Image	Note
TS11: trophoblast giant cell	Present	Regionally restricted	5A	Expression was detected in the parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Present	Regionally restricted	5B	Expression was detected in the parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Strong	Regionally restricted	5C	Expression was intense in trophoblast giant cells associated with the arterial side of the maternal blood circulation within the placenta.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Strong	Regionally restricted	5D	Intense expression was detected in all the cells of the developing junctional zone.

Structure	Level	Pattern	Image	Note
TS17: trophoblast giant cell	Present	Regionally restricted		Expression was detected in the parietal trophoblast giant cells.
TS17: trophectoderm	Strong	Regionally restricted		Intense expression was detected in all the cells of the developing junctional zone.

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Strong	Regionally restricted	5E	Expression was strong in the venous channel trophoblast giant cells, which span through the junctional zone.

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Present	Regionally restricted	5F	Expression was detected in the parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Strong	Regionally restricted	5G	Expression was intense in spiral artery trophoblast giant cells and arterial canal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Present	Regionally restricted	5H	Expression was detected in trophoblast giant cells located on the border between the labyrinth and junctional zone. Expression in glycogen trophoblast cells was less strong than at E9.5, and seemed most intense in the cells located on the basal side of the junctional zone.
TS20: spongiotrophoblast	Present		5H	Expression was less strong than at E9.5, and seemed most intense in the cells located on the basal side of the junctional zone.