Antibody:	anti-NCAM [H28.123]
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS21: epidermis	Absent		1D
TS21: dermis	Present		1D	Moderate-to-intense expression on all mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS21: epidermis	Absent		1E
TS21: dermis	Present	Patchy	1E	included a few intensely stained axonal sections

Structure	Level	Pattern	Image	Note
TS22: epidermis	Absent		2E
TS22: dermis	Weak	Patchy	2E	Only the upper mesenchyme sometimes showed patches of staining.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Absent		2F
TS22: dermis	Strong	Regionally restricted	2F	Staining was restricted to somitic cells and a layer of dermal myoblasts.

Structure	Level	Pattern	Image	Note
TS22: dermis	Strong	Regionally restricted	2G	Expressed in several axonal sections in the upper dermis.

Structure	Level	Pattern	Image	Note
TS23: hair epidermal placode	Present	Non-Uniform	3D	Expressed in most epithelial cells of the hair plugs. Staining intensity ranged from almost negative on the short, anterior side to very bright on the longer, posterior side of the hair plug.
TS23: dermis	Present	Regionally restricted	3D	Weak expression in a few cells just below the dermal epithelial junction. Bright staining in several axonal sections.

Structure	Level	Pattern	Image	Note
TS23: hair epidermal placode	Present	Regionally restricted	3E	Usually only the outer cells were stained, whereas the cells in the center and those facing the dermal cell condensation were negative.

Structure	Level	Pattern	Image	Note
TS25: hair epidermal placode	Strong	Regionally restricted	5D	Expressed in the upper caudal epithelium.
TS25: integumental system gland	Present	Regionally restricted	5D	Expressed in the sebaceous gland anlage.
TS25: hair root sheath	Present	Regionally restricted	5D	Expressed in the mesenchymal sheath.
TS25: hair follicle dermal papilla	Present		5D
TS25: dermis	Moderate	Regionally restricted	5D	Expressed in the interfollicular mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: hair root sheath	Present	Regionally restricted	5E	Expressed in the mesenchymal sheath.
TS25: hair follicle dermal papilla	Present		5E
TS25: dermis	Present	Regionally restricted	5E	Expressed in the interfollicular mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: hair follicle dermal papilla	Present		6F
TS26: hair root sheath	Present		6F
TS26: hair follicle	Present		6F	Expressed in the mesenchymal sheath and dermal papilla of the tylotrich follicle, elongated awl follicles with increased staining in the upper follicle epithelium and awl hair plug.
TS26: hair epidermal placode	Very strong	Regionally restricted	6F	Staining was negative in awl placodes and many awl hair plugs, but very intense in the upper epithelium of elongating awl follicles.

Structure	Level	Pattern	Image	Note
TS27: hair outer root sheath	Present	Regionally restricted	7C	Expressed in the upper outer root sheath above the sebaceousg land anlage.

Structure	Level	Pattern	Image	Note
TS27: hair follicle dermal papilla	Present
TS27: hair root sheath	Present	Regionally restricted		Expressed in the mesenchymal sheath.
TS27: hair epidermal placode	Moderate	Regionally restricted		Expressed in the zigzag placodes.