Probe:	Otx2 cDNA11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present	Regionally restricted	1H' , 1H	Expression was detected in the presumptive outer nuclear layer and in scattered cells throughout the outer neuroblastic layer.
TS20: pigmented retinal epithelium	Present		1H , 1H'

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	1I , 1I'	Expression was detected in the presumptive outer nuclear layer and in scattered cells throughout the outer neuroblastic layer. Additional expression was also detected in the inner neuroblast layer.
TS23: pigmented retinal epithelium	Present		1I , 1I'

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present		1J' , 1J	Expression peaked at P0.
TS27: pigmented retinal epithelium	Present		1J , 1J'
TS27: retina inner nuclear layer	Present	Regionally restricted	1J , 1J'	Expression was also detected in the inner neuroblast layer.

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Present	Scattered	1K' , 1K
TS28: retina outer nuclear layer	Present	Scattered	1K' , 1K
TS28: retina inner nuclear layer	Present	Regionally restricted	1K' , 1K	By P7 predominant expression was detected in bipolar cells in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Present	Scattered	1L' , 1L
TS28: retina outer nuclear layer	Present	Scattered	1L' , 1L
TS28: retina inner nuclear layer	Present	Regionally restricted	1L , 1L'	Predominant expression was detected in bipolar cells in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Present	Scattered	1M' , 1M
TS28: retina outer nuclear layer	Present	Scattered	1M' , 1M
TS28: retina inner nuclear layer	Present	Regionally restricted	1M' , 1M	Predominant expression was detected in bipolar cells in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Present	Scattered	1N , 1N'
TS28: retina outer nuclear layer	Present	Scattered	1N , 1N'
TS28: retina inner nuclear layer	Present	Regionally restricted	1N , 1N'	Predominant expression was detected in bipolar cells in the inner nuclear layer.