Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52362
Skowron JF, et al., Cloning and characterization of mouse brush border myosin-I in adult and embryonic intestine. J Exp Zool. 1999 Feb 15;283(3):242-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6194650
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Gene symbol:
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Myo1a
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Gene name:
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myosin IA
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Antibody:
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BBM-I antibody
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
5a:
postnatal adult
(more  )
Note: After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
Specimen
5a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine epithelium |
Present |
Regionally restricted |
5a
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Expression is at the apical domain of epithelial cells and is highest in cells above the crypt-villus transitional zone and is lower in crypts.
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Specimen
5c:
postnatal adult
(more )
Note: After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
Specimen
5c:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine epithelium |
Present |
Regionally restricted |
5c
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Expression is at the apical domain of epithelial cells and is highest in cells above the crypt-villus transitional zone and is lower in crypts and is absent in the terminal web region.
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Specimen
5e:
postnatal adult
(more )
Note: After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
Specimen
5e:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine epithelium |
Present |
Regionally restricted |
5e
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Expression is at the apical domain of epithelial cells and is lower in cells at earlier stages of differentiation down the crypt-villus axis. Undifferentiated enterocytes have the lowest expression at the basolateral membrane.
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Specimen
6a:
postnatal adult
(more )
Note: After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
Specimen
6a:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Cryosection
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|
Note:
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After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: colon epithelium |
Weak |
Regionally restricted |
6a
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Expression is in the apical domain and at the brush border and is excluded in the terminal web region.
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Specimen
6c:
postnatal adult
(more )
Note: After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
Specimen
6c:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: colon epithelium |
Weak |
Regionally restricted |
6c
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Expression is in the apical domain and at the brush border and is excluded in the terminal web region.
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Specimen
10a:
embryonic day 17.0
(more )
Note: After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
Specimen
10a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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After cryosectioning the tissues were re-fixed in acetone. Double labeled: green - Myo1a; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: small intestine epithelium |
Present |
Regionally restricted |
10a
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Expression is at the cell periphery; apical epithelium and basolateral membranes. In cells that have lower expression the staining is punctate and cytoplasmic.
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Analysis Tools