Gene Expression Data
Assay Details
Assay
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Reference:
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J:210661
Seto Y, et al., Characterization of Olig2 expression during cerebellar development. Gene Expr Patterns. 2014 May;15(1):1-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6194375
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Gene symbol:
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Olig2
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Gene name:
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oligodendrocyte transcription factor 2
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Results
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Specimen
2A,B,C:
embryonic day 12.5
(more )
Note: Double labeled: green - Olig2; magenta - Ptf1a (assay MGI:6194382).
Specimen
2A,B,C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Olig2; magenta - Ptf1a (assay MGI:6194382).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
2A
,
2B
,
2C
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Some of the Olig2 immunopositive cells were also labeled with Ptf1a, a marker for GABAergic neuron progenitors.
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Specimen
3A,G:
embryonic day 12.5
(more )
Note: Triple labeled: green - Olig2; blue - Ptf1a (assay MGI:6194387); red - Lhx1 (assay MGI:6194383) and Lhx5 (assay MGI:6194385).
Specimen
3A,G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Olig2; blue - Ptf1a (assay MGI:6194387); red - Lhx1 (assay MGI:6194383) and Lhx5 (assay MGI:6194385).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
3G
,
3A
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Triple labeling indicated that some Olig2-positive cells express Ptf1a (marker for GABAergic neuron progenitors) and other Olig2-positive cells express Lhx1 and Lhx5 (which mark postmitotic GABAergic neurons). The results indicate that Olig2 is expressed in both mitotic and postmitotic cells.
|
Specimen
3B,H:
embryonic day 12.5
(more )
Note: Double labeled: green - Olig2; magenta - Lhx1 (assay MGI:6194384) and Lhx5 (assay MGI:6194386).
Specimen
3B,H:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Olig2; magenta - Lhx1 (assay MGI:6194384) and Lhx5 (assay MGI:6194386).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
3H
,
3B
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Some Olig2-positive cells express Lhx1 and Lhx5 (which mark postmitotic GABAergic neurons).
|
Specimen
3D:
embryonic day 12.5
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
3I:
embryonic day 12.5
(more )
Note: Double labeled: green - Olig2; magenta - Ptf1a (assay MGI:6194382).
Specimen
3I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Olig2; magenta - Ptf1a (assay MGI:6194382).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
3I
|
Some Olig2-positive cells express Ptf1a (marker for GABAergic neuron progenitors).
|
Specimen
6D,E:
embryonic day 12.5
(more )
Note: Double labeled: green - Olig2; magenta - Skor2 (assay MGI:6194657).
Specimen
6D,E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Olig2; magenta - Skor2 (assay MGI:6194657).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
6E
,
6D
|
Olig2 expression was distinct from that of Skor2, an early marker of Purkinje cells.
|
Specimen
6G,H:
embryonic day 12.5
(more )
Note: Double labeled: green - Olig2; magenta - Skor2 (assay MGI:6194657).
Specimen
6G,H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Olig2; magenta - Skor2 (assay MGI:6194657).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
6H
,
6G
|
Olig2 expression was distinct from that of Skor2, an early marker of Purkinje cells.
|
|