Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Fbxo2tm1.1(cre/ERT2)Hels
Name:	F-box protein 2; targeted mutation 1.1, Stefan Heller
MGI ID:	MGI:6194328
Gene:	Fbxo2  Location: Chr4:148245078-148250881 bp, + strand  Genetic Position: Chr4, 78.68 cM
Alliance:	Fbxo2tm1.1(cre/ERT2)Hels page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Fbxo2tm1.1(cre/ERT2)Hels expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Fbxo2 (MGI:2446216)   Mutation details: The targeting vector was designed to insert a multicistronic reporter into the ATG start codon of the F-box protein 2 (Fbxo2) gene. Specifically, this vector includes (from 5' to 3') an H2B-Venus fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] with a C-terminal yellow fluorescent protein sequence), a hygromycin resistance gene flanked with viral P2A oligopeptides, a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain), a polyadenylation signal, and a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14tg2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Tg(EIIa-cre)C5379Lmgd mice on an FVB genetic background to remove the neo cassette. (J:101977)

Activity:	Tissue activity of this recombinase allele
Driver:	Fbxo2 (mouse) Summary of all recombinase alleles driven by Fbxo2. Alliance mouse Fbxo2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fbxo2 Mutation:	20 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	2 reference(s)