Gene Expression Data
Assay Details
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Assay
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Reference:
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J:137275
Yu Z, et al., Grainyhead-like factor Get1/Grhl3 regulates formation of the epidermal leading edge during eyelid closure. Dev Biol. 2008 Jul 1;319(1):56-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6194318
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Gene symbol:
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Tgfa
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Gene name:
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transforming growth factor alpha
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Antibody:
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TGFalpha antibody
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
7B:
embryonic day 14.0
(more  )
Specimen
7B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: eyelid epithelium |
Strong |
Regionally restricted |
7B
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Highest expression in most superficial keratinocytes of epidermis, including tip of eyelids.
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Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Grhl3tm1Bogi/Grhl3tm1Bogi
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: eyelid epithelium |
Weak |
Regionally restricted |
7C
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Lower levels of expression, especially decreased at tip of eyelid, where normal leading edge forms.
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Specimen
7D:
embryonic day 14.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: eyelid epithelium |
Strong |
Regionally restricted |
7D
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Highest expression in most superficial keratinocytes of epidermis, including tip of eyelids.
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Specimen
7E:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Grhl3tm1Bogi/Grhl3tm1Bogi
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: eyelid epithelium |
Weak |
Regionally restricted |
7E
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Lower levels of expression, especially decreased at tip of eyelid, where normal leading edge forms.
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Specimen
7F:
embryonic day 15.0
(more )
Specimen
7F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: eyelid epithelium |
Strong |
Regionally restricted |
7F
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Highest expression in leading edge of eyelids.
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Specimen
7G:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Grhl3tm1Bogi/Grhl3tm1Bogi
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: eyelid epithelium |
Weak |
Regionally restricted |
7G
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Lower levels of expression, especially decreased at tip of eyelid, where normal leading edge forms.
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Specimen
7H:
embryonic day 15.5
(more )
Specimen
7H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: eyelid epithelium |
Strong |
Regionally restricted |
7H
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Highest expression in leading edge of eyelids.
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Specimen
7I:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Grhl3tm1Bogi/Grhl3tm1Bogi
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: eyelid epithelium |
Weak |
Regionally restricted |
7I
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Lower levels of expression, especially decreased at tip of eyelid, where normal leading edge forms.
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Analysis Tools