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Gene Expression Data
Assay Details
Assay
Reference: J:137275 Yu Z, et al., Grainyhead-like factor Get1/Grhl3 regulates formation of the epidermal leading edge during eyelid closure. Dev Biol. 2008 Jul 1;319(1):56-67
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6194317
Gene symbol: Grhl3
Gene name: grainyhead like transcription factor 3
Antibody: Anti-Get1
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: eyelid epithelium Present Uniform 2A Uniform expression in epidermal keratinocytes covering eyelid.
Specimen 2B: embryonic day 14.5 (more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Structure Level Pattern Image Note
TS22: eyelid epithelium Strong Regionally restricted 2B Expression upregulated in cluster of rounded keratinocytes initiating formation of leading edge.
Specimen 2C: embryonic day 14.5 (more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Structure Level Pattern Image Note
TS22: eyelid epithelium Strong Regionally restricted 2C Expression upregulated in cluster of rounded keratinocytes initiating formation of leading edge.
Specimen 2E: embryonic day 15.5 (more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Structure Level Pattern Image Note
TS23: eyelid epithelium Strong Regionally restricted 2E High expression in keratinocytes of outer layer of migrating edge.
Specimen 2F: embryonic day 15.5 (more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Structure Level Pattern Image Note
TS23: eyelid epithelium Strong Regionally restricted 2F High expression in keratinocytes of outer layer of migrating edge.
Specimen 2D: embryonic day 17.0 (more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Structure Level Pattern Image Note
TS25: eyelid epithelium Weak Regionally restricted 2D Expression downregulated along fusion line but persists in suprabasal epidermal keratinocytes covering eyelids.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory