Antibody:	Anti-Olig2 (AF2418)
Assay notes:	Two antibodies were described; the one used in this assay was not detailed. Either MGI:6194277 or MGI:6194278 could have been used.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1A	Expression was detected in the ventral telencephalon.
TS21: cerebellum	Present	Regionally restricted	1D , 1A	There are two distinct Olig2-positive populations in the cerebellum. One is near the pial surface of the cerebellum, and a second signal is near the 4th ventricle.
TS21: cerebellum ventricular layer	Present		1D , 1A

Structure	Level	Pattern	Image	Note
TS18: cerebellum primordium	Absent		1B

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present		1C

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	1E	There are two distinct Olig2-positive populations in the cerebellum. One is near the pial surface of the cerebellum, and a second signal is near the 4th ventricle.
TS21: cerebellum ventricular layer	Present		1E

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	1F	Expression was detected near the pial surface of the cerebellum.
TS22: cerebellum ventricular layer	Absent		1F

Structure	Level	Pattern	Image	Note
TS24: deep cerebellar nucleus	Present	Regionally restricted	1H , 1G	Expression was detected in the interposed and lateral deep cerebellar nuclei. High magnification of double staining showed cells that were immunopositive for both Olig2 and Pou3f2, as well as other cells that were only Olig2-positive.

Structure	Level	Pattern	Image	Note
TS24: deep cerebellar nucleus	Present	Regionally restricted	1K , 1J	Expression was detected in the interposed and lateral deep cerebellar nuclei. There was no overlap of staining with Tbr1 in the medial deep nuclei.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	2E , 2G , 2F	Some of the Olig2 immunopositive cells were also labeled with Mki67, a mitotic cell marker.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	2I , 2J , 2K	Some of the Olig2 immunopositive cells were also labeled with BrdU, which labels cells undergoing DNA synthesis.