Antibody:	PKD2-NP
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: outer renal medulla	Present		4b	A clear demarcation of expression can be seen between the cortex and the outer medulla.
TS28: renal cortex	Present		4b	A clear demarcation of expression can be seen between the cortex and the outer medulla.

Structure	Level	Pattern	Image	Note
TS28: renal distal tubule	Strong	Regionally restricted	4c	Strong basolateral staining was seen in the distal tubules.
TS28: glomerular tuft	Absent		4c
TS28: renal proximal convoluted tubule	Weak	Regionally restricted	4c	Occasional weak basolateral expression was detected in the proximal convoluted tubules.

Structure	Level	Pattern	Image	Note
TS28: renal distal tubule	Strong	Regionally restricted	4d	Strong basolateral staining was seen in the distal tubules.
TS28: renal proximal convoluted tubule	Weak	Regionally restricted	4d	Occasional weak basolateral expression was detected in the proximal convoluted tubules.

Structure	Level	Pattern	Image	Note
TS28: distal straight tubule of renal cortex	Present	Regionally restricted	4e	Basolateral staining was seen in the thick ascending limb of the loop of Henle.
TS28: loop of Henle thin descending limb	Absent		4e

Structure	Level	Pattern	Image	Note
TS28: papillary duct	Present		4f
TS28: urothelium	Present		4f
TS28: renal distal tubule	Strong		4f

Structure	Level	Pattern	Image	Note
TS28: renal interstitium	Absent
TS28: renal vasculature	Absent

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Weak	Regionally restricted	5h	Weak staining of the ureteric bud was noted.
TS22: renal tubule	Weak	Regionally restricted	5h	Weak staining of the primitive tubules.
TS22: comma-shaped body	Absent		5h
TS22: s-shaped body	Absent		5h

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Absent