Gene Expression Data
Assay Details
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Assay
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Reference:
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J:226319
Phippard D, et al., Changes in the subcellular localization of the Brn4 gene product precede mesenchymal remodeling of the otic capsule. Hear Res. 1998 Jun;120(1-2):77-85
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6193861
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Gene symbol:
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Pou3f4
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Gene name:
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POU domain, class 3, transcription factor 4
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Antibody:
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Anti-Brn4
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Immunoperoxidase signal was amplified with the TSA Indirect Tyramide Signal Amplification Kit.
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Results
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Specimen
4A:
embryonic day 10.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
4A
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Expression was detected in the ventral aspect.
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| TS17: hindbrain |
Present |
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4A
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Specimen
4B,C:
embryonic day 11.5
(more )
Specimen
4B,C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5:
embryonic day 16.5
(more )
Specimen
5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cartilaginous otic capsule |
Present |
Regionally restricted |
5_Top panel
,
5A
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Cells in the cartilaginous shell of the otic capsule demonstrate a predominantly nuclear localization of protein.
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| TS24: inner ear |
Present |
Regionally restricted |
5_Top panel
,
5B
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Cells in the region of the future apical scala vestibuli demonstrate a perinuclear immunolocalization.
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| TS24: bony labyrinth |
Present |
Regionally restricted |
5_Top panel
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5C
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The majority of cells in the anlage of the modiolus demonstrate a nuclear staining pattern, although a few cells in this region demonstrate a perinuclear localization.
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Specimen
6A:
embryonic day 16.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cartilaginous otic capsule |
Present |
Regionally restricted |
6A
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Nuclear localization of expression was detected in the cartilaginous shell of the otic capsule.
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| TS24: temporal bone petrous part |
Present |
Regionally restricted |
6A
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Perinuclear localization of expression was detected in the anlage of the internal auditory meatus.
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Specimen
6B:
embryonic day 16.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: temporal bone petrous part |
Present |
Regionally restricted |
6B
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Perinuclear localization of expression was detected in the anlage of the internal auditory meatus.
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Specimen
6C:
embryonic day 14.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: bony labyrinth |
Present |
Regionally restricted |
6C
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Perinuclear localization of expression was detected in the future scala vestibuli.
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| TS22: inner ear mesenchyme |
Present |
Regionally restricted |
6C
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Nuclear localization of expression was detected in the mesenchyme adjacent to the otic vesicle.
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Specimen
6D:
embryonic day 14.5
(more )
Specimen
6D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: bony labyrinth |
Present |
Regionally restricted |
6D
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Perinuclear localization of expression was detected in the future scala tympani.
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| TS22: inner ear mesenchyme |
Present |
Regionally restricted |
6D
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Nuclear localization of expression was detected in the mesenchyme adjacent to the otic vesicle.
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Analysis Tools