Tg(KRT14)1Efu
Transgene Detail
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| Symbol: |
Tg(KRT14)1Efu |
| Name: |
transgene insertion 1, Elaine Fuchs |
| MGI ID: |
MGI:6192717 |
| Synonyms: |
K14-P |
| Transgene: |
Tg(KRT14)1Efu Location: unknown
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| Alliance: |
Tg(KRT14)1Efu page
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| Transgene Type: |
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Transgenic (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(KRT14)1Efu expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
KRT14 (3861) |
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human promoter and gene |
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Mutation details: The transgene comprises the following elements, all from human keratin 14: upstream sequence (from the HindIII site 2.5 kb upstream of the transcription start site) including the promoter, the cDNA with the antigenic portion of neuropeptide tachykinin 1 (substance P) inserted in-frame 18 bp upstream of the stop codon, and 3' UTR sequence including poly(A) signal (up to the EcoRI site 700 bp downstream of poly(A) signal). The construct was micro-injected into the male pronuclei of fertilized oocytes. Immunohistological analysis using substance P antibody shows expression in the basal layer of the epidermis and in the outer root sheath cells of the hair follicles. Line 1 showed consistent (non-chimeric) expression of the transgene.
(J:113642)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:113642 Vassar R, et al., Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1563-7 |
| All: |
1 reference(s) |
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Analysis Tools
