Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1(CAG-EGFP)Brsy
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kivanc Birsoy
MGI ID:	MGI:6192094
Synonyms:	MITO-Tag, Rosa26LSL-3XHA-EGFP-OMP25
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1(CAG-EGFP)Brsy page

Germline Transmission:	Earliest citation of germline transmission: J:268121
Parent Cell Line:	CY2.4 (ES Cell)
Strain of Origin:	B6(Cg)-Tyrc-2J

Allele Type:    Targeted (Conditional ready, Epitope tag, Reporter) Mutation:    Insertion   Mutation details: The targeting construct containing (from 5' t 3') CAG-loxP-neomycin (reversed)-pA-loxP-3xHA-EGFP-SYNJ2BP-lacZ-BGH-pA is targeted to intron 1 of the gene. The 3xHA-EGFP-SYNJ2BP components constitute the "MITO-Tag" cassette targeting the 3XHA epitope tag with GFP to the mitochondrial membrane. The construct includes only the transmembrane domain of the rat Synj2bp/OMP25 (AA109-145) gene. The small fragment of lacZ incorporated in the mutation is not expected to be functional. (J:268121)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:268121 Bayraktar EC, et al., MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo. Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):303-312
All:	1 reference(s)