Gene Expression Data
Assay Details
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Assay
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Reference:
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J:132608
Waimey KE, et al., Plexin-A3 and plexin-A4 restrict the migration of sympathetic neurons but not their neural crest precursors. Dev Biol. 2008 Mar 15;315(2):448-58
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6191715
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2A:
embryonic day 13.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Plxna3tm1Matl/Plxna3tm1Matl
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Plxna4tm1Matl/Plxna4tm1Matl
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2D:
(close )
Specimen
2E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Plxna4tm1Matl/Plxna4tm1Matl
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2F:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: sympathetic nervous system |
Present |
Regionally restricted |
2F
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Authors report a greater number of fasciculating axons in double mutant than in Plxna4 single mutant.
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Specimen
2G:
(close )
Specimen
2H:
embryonic day 13.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: sympathetic ganglion |
Present |
Regionally restricted |
2H
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Expression in sympathetic chain ganglia at trunk levels.
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Plxna3tm1Matl/Plxna3tm1Matl
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2J:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: sympathetic ganglion |
Present |
Regionally restricted |
2J
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Authors report defects at trunk levels in Plxna3/Plxna4 double mutants.
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Specimen
3P:
embryonic day 10.5
(more )
Specimen
3P:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3Q:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Nrp2tm1Ddg/Nrp2tm1Ddg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: sympathetic nervous system |
Present |
Regionally restricted |
3Q
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Authors report normal aggregation occurs in double mutants; no ectopic cells are seen.
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Analysis Tools