Antibody:	anti-nesfatin-1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present		1a
TS11: extraembryonic ectoderm	Present		1a

Structure	Level	Pattern	Image	Note
TS28: uterus	Present	Regionally restricted	1a	Expressed in the uterine tissue immediately adjacent to the developing placenta.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic ectoderm	Present		1b
TS11: trophoblast giant cell	Present		1b

Structure	Level	Pattern	Image	Note
TS11: extraembryonic ectoderm	Present		1c
TS11: trophoblast giant cell	Present		1c

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Strong		2a
TS13: chorionic plate	Present		2a	Much less expression than the parietal trophoblast giant cells.
TS13: extraembryonic ectoderm	Present		2a

Structure	Level	Pattern	Image	Note
TS28: uterus	Present	Regionally restricted	2a	Reduced expression in the uterine parenchyma immediately adjacent to the embryo.

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Strong		2b	Expressed in in the majority but not all.
TS13: ectoplacental cone	Strong		2b
TS13: chorionic plate	Present		2b	Much less expression than the parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Strong		2c	Expressed in in the majority but not all.
TS13: ectoplacental cone	Strong		2c
TS13: chorionic plate	Present		2c	Much less expression than the parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Strong		3a
TS15: spongiotrophoblast	Present		3a

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Strong		3b
TS15: spongiotrophoblast	Present		3b

Structure	Level	Pattern	Image	Note
TS15: labyrinthine zone	Strong		3c	Particularly in the fetal capillary endothelial cells.

Structure	Level	Pattern	Image	Note
TS17: trophoblast giant cell	Strong		4a
TS17: labyrinthine zone	Present		4a
TS17: spongiotrophoblast	Present		4a

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		4a

Structure	Level	Pattern	Image	Note
TS17: trophoblast giant cell	Strong		4b
TS17: labyrinthine zone	Present		4b
TS17: spongiotrophoblast	Present		4b

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		4b

Structure	Level	Pattern	Image	Note
TS17: labyrinthine zone	Present		4c	Expression was particularly prominent in fetal endothelial cells.

Structure	Level	Pattern	Image	Note
TS20: labyrinthine zone	Strong		5a
TS20: trophoblast giant cell	Strong		5a
TS20: spongiotrophoblast	Trace		5a	Very low level.

Structure	Level	Pattern	Image	Note
TS20: labyrinthine zone	Strong		5b
TS20: spongiotrophoblast	Trace		5b	Very low level.
TS20: trophoblast giant cell	Strong		5b	Expressed in the glycogen trophoblast cells.

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Present		5c	Expressed in the sinusoidal trophoblast giant cells.
TS20: labyrinthine zone	Present		5c	Expressed in the fetal capillary endothelial cells and labyrinthine trophoblast cells.

Structure	Level	Pattern	Image	Note
TS23: labyrinthine zone	Strong		6a

Structure	Level	Pattern	Image	Note
TS23: trophoblast giant cell	Present		6b	Considerable expression in glycogen trophoblast cells, but very low expression in the parietal trophoblast giant cells.
TS23: spongiotrophoblast	Trace		6b	Very low level.

Structure	Level	Pattern	Image	Note
TS23: trophoblast giant cell	Present		6c	Expressed in the maternal sinusoidal trophoblast giant cells.
TS23: labyrinthine zone	Present		6c	Expressed in the fetal capillary endothelial cells.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		S1A
TS20: yolk sac	Present		S1A

Structure	Level	Pattern	Image	Note
TS28: placenta	Present		S1A