Gene Expression Data
Assay Details
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Assay
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Reference:
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J:131010
Kameda Y, et al., FRS2 alpha 2F/2F mice lack carotid body and exhibit abnormalities of the superior cervical sympathetic ganglion and carotid sinus nerve. Dev Biol. 2008 Feb 1;314(1):236-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6189175
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Gene symbol:
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Uchl1
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Gene name:
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ubiquitin carboxy-terminal hydrolase L1
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Antibody:
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Anti-PGP9.5
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixation was Bouin's or 8% paraformaldehyde.
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Results
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Specimen
1C:
embryonic day 18.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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involves: Swiss Webster
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: carotid body |
Present |
Regionally restricted |
1C
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Expression in glomus cells and neurons of the superior cervical ganglion.
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| TS26: superior cervical ganglion |
Present |
Regionally restricted |
1C
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Expression in glomus cells and neurons of the superior cervical ganglion.
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S/SvEv * Swiss Webster
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Mutant Allele(s):
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Frs2tm1Schl/Frs2tm1Schl
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: superior cervical ganglion |
Present |
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4B
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| TS26: carotid body |
Present |
Regionally restricted |
4B
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Authors report expression in glomus cells dispersed in the wall of the common carotid artery where baroreceptors were distributed and sympathetic ganglion abutted.
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Specimen
5A:
embryonic day 12.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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involves: Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: sympathetic ganglion |
Present |
Regionally restricted |
5A
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Authors report an elongated immature sympathetic ganglion is located along the dorsal aorta.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129S/SvEv * Swiss Webster
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Mutant Allele(s):
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Frs2tm1Schl/Frs2tm1Schl
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inferior cervical ganglion |
Present |
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5C
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Authors state the immature superior cervical ganglion was similar to wild type embryos, and clearly separated from the stellate (inferior cervical) ganglion.
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| TS21: superior cervical ganglion |
Present |
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5C
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Authors state the immature superior cervical ganglion was similar to wild type embryos, and clearly separated from the stellate (inferior cervical) ganglion.
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| TS21: carotid artery |
Absent |
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5C
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Carotid body rudiment was not formed in the wall of the third arch artery.
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Analysis Tools