Gene Expression Data
Assay Details
Assay
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Reference:
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J:89383
Winston NJ, et al., Expression and role of the ether-a-go-go-related (MERG1A) potassium-channel protein during preimplantation mouse development. Biol Reprod. 2004 Apr;70(4):1070-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6188886
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Gene symbol:
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Kcnh2
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Gene name:
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potassium voltage-gated channel, subfamily H (eag-related), member 2
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Antibody:
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C-anti-HERG
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
5A:
postnatal adult
(more )
Note: Metaphase-arrested oocyte
Specimen
5A:
(close )
Genetic Background:
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Swiss
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Metaphase-arrested oocyte
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Structure |
Level |
Pattern |
Image |
Note |
TS28: primary oocyte |
Present |
Regionally restricted |
5A
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Expression is in the cytoplasm around the spindle, especially around the equator.
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Specimen
5B:
embryonic day 0.5
(more )
Note: G1- phase 1-cell stage
Specimen
5B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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G1- phase 1-cell stage
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Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
|
5B
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Expression is centrally in the cytoplasm and is excluded form the pronuclei.
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Specimen
5C:
embryonic day 0.5
(more )
Note: S-phase 1-cell stage
Specimen
5C:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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S-phase 1-cell stage
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Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
|
5C
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Expression is in the cytoplasm and in the expanded pronuclei as a ring around the nucleoli.
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Specimen
5D:
embryonic day 1.0
(more )
Note: Early 2-cell stage embryo.
Specimen
5D:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early 2-cell stage embryo.
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Specimen
5E:
embryonic day 1.5
(more )
Note: Late 2-cell stage embryo.
Specimen
5E:
(close )
Genetic Background:
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Swiss
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Age:
|
embryonic day 1.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late 2-cell stage embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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5E
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Expression is increasingly located in the nucleus and the nuclear and plasma membranes and is weaker in the cytoplasm.
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Specimen
5F:
embryonic day 2.0
(more )
Note: 4-cell stage embryo.
Specimen
5F:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-cell stage embryo.
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Specimen
5G:
embryonic day 2.0
(more )
Note: 8-cell stage embryo.
Specimen
5G:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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8-cell stage embryo.
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Specimen
5H:
embryonic day 2.5
(more )
Note: Morula-cell stage embryo.
Specimen
5H:
(close )
Genetic Background:
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Swiss
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Age:
|
embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Morula-cell stage embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
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5H
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Expression is basolateral at the plasmamembrane.
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Specimen
5I:
embryonic day 3.5
(more )
Note: Blastocyst-cell stage embryo.
Specimen
5I:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Blastocyst-cell stage embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS4: inner cell mass |
Present |
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5I
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Expression is basolateral at the plasmamembrane.
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Specimen
6A:
postnatal adult
(more )
Note: Metaphase of meiosis II oocyte.
Specimen
6A:
(close )
Genetic Background:
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Swiss
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Metaphase of meiosis II oocyte.
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Specimen
6B:
embryonic day 0.5
(more )
Note: 1-cell stage embryo.
Specimen
6B:
(close )
Genetic Background:
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Swiss
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Age:
|
embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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1-cell stage embryo.
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Specimen
6C:
embryonic day 1.0
(more )
Note: 2-cell stage embryo.
Specimen
6C:
(close )
Genetic Background:
|
Swiss
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Age:
|
embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-cell stage embryo.
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Specimen
6D:
embryonic day 2.0
(more )
Note: 4-cell stage embryo.
Specimen
6D:
(close )
Genetic Background:
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Swiss
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Age:
|
embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-cell stage embryo.
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