Probe:	PPET-1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: 1st arch artery	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: 2nd arch artery	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: 3rd arch artery	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: aortic sac	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: cardinal vein	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: carotid artery	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: dorsal aorta	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: outflow tract	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: umbilical blood vessel	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.
TS15: vitelline blood vessel	Strong	Regionally restricted	2A/B	Expressed in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS17: intersomitic blood vessel	Present	Regionally restricted	2C/D	Expressed in the endothelial cells.
TS17: dorsal aorta	Present	Regionally restricted	2C/D	Expressed in the endothelial cells.
TS17: endocardial lining	Present		2C/D
TS17: pericardium	Present		2C/D

Structure	Level	Pattern	Image	Note
TS19: primitive meninges	Present	Regionally restricted	2E/F	Expressed in the meningeal vessels surrounding the embryonic brain and spinal cord.
TS19: dorsal aorta	Present	Regionally restricted	2E/F	Expressed in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS21: intersomitic blood vessel	Present	Regionally restricted	3A/B	Expressed in the endothelial cells.
TS21: brain meninges	Present	Regionally restricted	3A/B	Expressed in the meningeal vessels.
TS21: spinal cord meninges	Present	Regionally restricted	3A/B	Expressed in the meningeal vessels.

Structure	Level	Pattern	Image	Note
TS21: endocardial lining	Present		3C/D	Much higher expression than that of E10.5.
TS21: pericardium	Present		3C/D	Much higher expression than that of E10.5.

Structure	Level	Pattern	Image	Note
TS21: lung parenchyma	Present		4A/B
TS21: lung vascular element	Present		4A/B

Structure	Level	Pattern	Image	Note
TS26: lung parenchyma	Present	Regionally restricted	4C/D	Strongest expression. Primarily in the endothelial cells.
TS26: lung vascular element	Present	Regionally restricted	4C/D	Strongest expression. Primarily in the endothelial cells.
TS26: pulmonary artery	Present	Regionally restricted	4C/D	Expressed in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Strong	Regionally restricted	5A	Expressed in the epithelium.
TS15: 2nd branchial arch	Strong	Regionally restricted	5A	Expressed in the epithelium.
TS15: 3rd branchial arch	Strong	Regionally restricted	5A	Expressed in the epithelium.
TS15: 1st branchial arch mesenchyme	Trace		5A
TS15: 2nd branchial arch mesenchyme	Trace		5A
TS15: 3rd branchial arch mesenchyme	Trace		5A
TS15: branchial arch artery	Strong	Regionally restricted	5A	Expressed in the aortic arch endothelial cells.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Weak	Regionally restricted	5B	Expressed in the branchial epithelium.
TS17: 2nd branchial arch	Weak	Regionally restricted	5B	Expressed in the branchial epithelium.
TS17: 3rd branchial arch	Weak	Regionally restricted	5B	Expressed in the branchial epithelium.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present		6A/B

Structure	Level	Pattern	Image	Note
TS20: lens vesicle anterior epithelium	Very strong		6C/D	Expressed in the cuboidal epithelium.
TS20: lens vesicle posterior epithelium	Present		6C/D	Weaker expression.
TS20: cornea	Strong	Regionally restricted	6C/D	Expressed in the corneal ectoderm.
TS20: retina	Absent		6C/D
TS20: eye	Present	Regionally restricted	6C/D	No expression in the choroid and sclera.

Structure	Level	Pattern	Image	Note
TS17: optic cup	Present	Regionally restricted	6E/F	Expressed in a focal region in the epithelium.

Structure	Level	Pattern	Image	Note
TS21: cochlea	Strong	Regionally restricted	6G/H	Expressed in the focal region of the cochlea, mostly concentrated in the basilar papilla.

Structure	Level	Pattern	Image	Note
TS21: intestine epithelium	Very strong		7A/B

Structure	Level	Pattern	Image	Note
TS26: stomach glandular region epithelium	Present		7D , 7C
TS26: stomach glandular region lamina propria	Present		7D , 7C

Structure	Level	Pattern	Image	Note
TS26: intestine lamina propria	Weak		7E
TS26: intestinal villus	Weak	Regionally restricted	7E	Expressed in the lamina propria. Barely expressed in the epithelial cells.

Structure	Level	Pattern	Image	Note
TS17: midgut epithelium	Present
TS17: esophagus	Absent
TS17: stomach	Absent

Structure	Level	Pattern	Image	Note
TS19: lung vascular element	Present

Structure	Level	Pattern	Image	Note
TS20: optic stalk	Present

Structure	Level	Pattern	Image	Note
TS21: stomach	Weak

Structure	Level	Pattern	Image	Note
TS26: myenteric nerve plexus	Absent