Gene Expression Data
Assay Details
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Assay
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Reference:
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J:245222
Wilcox SM, et al., The role of the innate immune response regulatory gene ABCF1 in mammalian embryogenesis and development. PLoS One. 2017;12(5):e0175918
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6187975
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Gene symbol:
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Abcf1
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Gene name:
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ATP-binding cassette, sub-family F member 1
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Reporter:
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Beta-geo
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Direct Detection
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Assay notes:
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Fixative was 0.2% gluteraldehyde. Quantitative representation of adult sectioned tissues is shown in figure 6.
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Results
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Specimen
2A +/-:
(close  )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2A -/-:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1Gt(XK097)Byg
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3A:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3B:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
3C:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
4 Thymus:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Lymph node:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Lung:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Testis:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Brain:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Liver:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Heart:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Kidney:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Eye:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
4 Spleen:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
5A:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5B:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5C:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5D:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5E:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: liver |
Moderate |
Regionally restricted |
5E
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There is cytoplasmic staining in the peri-venular regions.
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Specimen
5F:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5G:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5H:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5I:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5J:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5K:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
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Mutant Allele(s):
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Abcf1Gt(XK097)Byg/Abcf1+
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|
Age:
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postnatal adult
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|
Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Glutaraldehyde
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Embedding:
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Cryosection
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Specimen
5L:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
|
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Mutant Allele(s):
|
Abcf1Gt(XK097)Byg/Abcf1+
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|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
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Type:
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section from whole mount
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|
Fixation:
|
Glutaraldehyde
|
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spleen |
Moderate |
Regionally restricted |
5L
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Expression is cytoplasmic in the marginal zone and little or no staining is seen in the periarticular lymphocyte area.
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| TS28: spleen marginal zone |
Moderate |
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5L
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Specimen
5M:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
|
|
Mutant Allele(s):
|
Abcf1Gt(XK097)Byg/Abcf1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
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|
Type:
|
section from whole mount
|
|
Fixation:
|
Glutaraldehyde
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|
Embedding:
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Cryosection
|
Specimen
5N:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
|
|
Mutant Allele(s):
|
Abcf1Gt(XK097)Byg/Abcf1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
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Type:
|
section from whole mount
|
|
Fixation:
|
Glutaraldehyde
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|
Embedding:
|
Cryosection
|
Specimen
not shown:
(close )
|
Genetic Background:
|
either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)
|
|
Mutant Allele(s):
|
Abcf1Gt(XK097)Byg/Abcf1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
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Analysis Tools
)