Probe:	Cnbp probe2
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: yolk sac mesoderm	Strong		4a , 4b	Highest concentration of expression in visceral yolk sac mesoderm and maternal decidua.
TS11: yolk sac endoderm	Present		4a , 4b	Highest concentration of expression in visceral yolk sac mesoderm and maternal decidua.

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		4a , 4b	Highest concentration of expression in visceral yolk sac mesoderm and maternal decidua.
TS28: blood	Absent		4b , 4a

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Widespread		Authors report expression in most other tissues at lower levels.
TS11: neural ectoderm	Absent			No expression in regions of future neural epithelia.

Structure	Level	Pattern	Image	Note
TS12: yolk sac mesenchyme	Strong		4c	Higher concentration of expression in visceral yolk sac mesoderm compared with visceral yolk sac endoderm.
TS12: yolk sac endoderm	Present		4c	Higher concentration of expression in visceral yolk sac mesoderm compared with visceral yolk sac endoderm.
TS12: surface ectoderm	Present		4c	Higher expression in surface ectoderm and cephalic mesenchyme in region of first branchial arch, compared with neural epithelium.
TS12: head mesenchyme	Present	Regionally restricted	4c	Higher expression in surface ectoderm and cephalic mesenchyme in region of first branchial arch, compared with neural epithelium.
TS12: neural ectoderm	Ambiguous		4c	Higher expression in surface ectoderm and cephalic mesenchyme in region of first branchial arch, compared with neural epithelium.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present	Regionally restricted	4d/e	Authors report expressing and non-expressing primitive erythroblasts throughout the yolk sac.
TS12: blood island	Ambiguous	Regionally restricted	4d/e	Authors state expression in blood islands was irregular.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Widespread		Authors report expression at levels above background in most embryonic tissues.
TS12: foregut diverticulum	Absent
TS12: hindgut diverticulum	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread	5a	Authors report expression in most embryonic tissues at this stage.
TS15: branchial arch	Strong		5a	Highest expression in surface ectoderm, hepatic primordia, branchial arches and posterior spine.
TS15: hepatic primordium	Strong		5a	Highest expression in surface ectoderm, hepatic primordia, branchial arches and posterior spine.
TS15: surface ectoderm	Strong		5a	Highest expression in surface ectoderm, hepatic primordia, branchial arches and posterior spine.
TS15: gut	Weak		5a	Reduced levels of expression in neuroepithelium, otic vesicle and gut.
TS15: neural ectoderm	Weak		5a	Reduced levels of expression in neuroepithelium, otic vesicle and gut.
TS15: otocyst	Weak		5a	Reduced levels of expression in neuroepithelium, otic vesicle and gut.

Structure	Level	Pattern	Image	Note
TS15: blood	Absent			No expression in primitive erythrocytes.

Structure	Level	Pattern	Image	Note
TS20: liver	Strong		5b/d	Highest expression in liver and lung.
TS20: lung	Strong		5b/d	Highest expression in liver and lung.
TS20: intestine mesenchyme	Strong	Regionally restricted	5b/d	Highest expression in lamina propria of intestine.
TS20: brain mantle layer	Present		5b/d	Authors report lower but significant levels of expression in mantle zone of nervous system.

Structure	Level	Pattern	Image	Note
TS22: embryo	Weak	Regionally restricted	5c	Authors state overall expression had decreased several fold when compared with earlier stages.
TS22: metanephros	Trace	Regionally restricted	5c	Tissues with higher than background expression include floor of lateral ventricle and developing kidney exclusive of glomeruli.
TS22: telencephalon	Trace	Regionally restricted	5c	Tissues with higher than background expression include floor of lateral ventricle and developing kidney exclusive of glomeruli.

Structure	Level	Pattern	Image	Note
TS20: lung	Strong		5f/g
TS20: somite	Present		5f/g	Authors report lower but significant levels of expression in somite.

Structure	Level	Pattern	Image	Note
TS20: intestine	Strong		5h/i
TS20: gonad	Present		5h/i	Authors report lower but significant levels of expression in gonad.