Antibody:	TROMA 1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct	Absent		3B	Rostral to growing tip, no expression in Mullerian duct.
TS20: nephric duct	Strong	Regionally restricted	3B	Expression in Wolffian duct localized to apical, lateral and basal regions of epithelial cells.
TS20: coelomic epithelium of reproductive system	Present		3B

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct	Absent		3G	At growing tip, no expression in Mullerian duct.
TS20: nephric duct	Strong	Regionally restricted	3G	Expression in Wolffian duct localized to apical, lateral and basal regions of epithelial cells.
TS20: coelomic epithelium of reproductive system	Present		3G

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct	Absent		3L	Caudal to growing tip, no expression in Mullerian duct.
TS20: nephric duct	Strong	Regionally restricted	3L	Expression in Wolffian duct localized to apical, lateral and basal regions of epithelial cells.
TS20: coelomic epithelium of reproductive system	Present		3L

Structure	Level	Pattern	Image	Note
TS21: paramesonephric duct of female	Absent		4B	In middle region, no expression in Mullerian duct.
TS21: nephric duct of female	Strong		4B	Expression in Wolffian duct
TS21: coelomic epithelium of reproductive system	Present		4B

Structure	Level	Pattern	Image	Note
TS21: paramesonephric duct of female	Absent		4H	In middle region, no expression in Mullerian duct.
TS21: nephric duct of female	Strong		4H	Expression in Wolffian duct
TS21: coelomic epithelium of reproductive system	Present		4H

Structure	Level	Pattern	Image	Note
TS21: paramesonephric duct of female	Absent		4N	In caudal region, no expression in Mullerian duct.
TS21: nephric duct of female	Strong		4N	Expression in Wolffian duct
TS21: coelomic epithelium of reproductive system	Present		4N