Antibody:	Anti-NeuN (clone A60; MAB377)
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present	Regionally restricted	4H , 4I	Expression was detected in post-mitotic neurons.

Structure	Level	Pattern	Image	Note
TS28: pons	Present		4L	Expression was detected in neurons. Rbfox3 and Calumenin expression co-localized in individual large neuronal cell bodies.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		4M	Expression was detected in the basal part of the granule cell layer. There were cells co-labeled with Calumenin.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Weak		4P	Co-expression of Rbfox3 and Calu was noted in a large cell in subgranular layer.
TS28: dentate gyrus granule cell layer	Present		4Q , 4P	A cell co-labeled with Calu and Rbfox3 was noted at the border between the granule cell layer and the molecular layer.
TS28: dentate gyrus molecular layer	Present		4Q , 4P	A cell co-labeled with Calu and Rbfox3 was noted at the border between the granule cell layer and the molecular layer.