Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119877
Quinn JC, et al., Pax6 controls cerebral cortical cell number by regulating exit from the cell cycle and specifies cortical cell identity by a cell autonomous mechanism. Dev Biol. 2007 Feb 1;302(1):50-65
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6160892
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Antibody:
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anti-Mash1
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Detection system:
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Alexa Fluor
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Results
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Specimen
8A:
embryonic day 12.5
(more  )
Note: Triple labeled: green - Mash1 (Ascl1); red - Tbr2 (Eomes assay MGI:6160891); blue - DNA.
Specimen
8A:
(close  )
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Genetic Background:
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involves: Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Mash1 (Ascl1); red - Tbr2 (Eomes assay MGI:6160891); blue - DNA.
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Specimen
8B:
embryonic day 12.5; Pax6Sey/ Pax6Sey
(more )
Note: Triple labeled: green - Mash1 (Ascl1); red - Tbr2 (Eomes assay MGI:6160891); blue - DNA.
Specimen
8B:
(close )
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Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Mash1 (Ascl1); red - Tbr2 (Eomes assay MGI:6160891); blue - DNA.
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Specimen
8C:
embryonic day 14.5
(more )
Note: Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
Specimen
8C:
(close )
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Genetic Background:
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involves: Swiss
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
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Specimen
8D:
embryonic day 14.5; Pax6Sey/ Pax6Sey
(more )
Note: Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
Specimen
8D:
(close )
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Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Widespread |
8D
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Expression in dorsal and ventreal telencephalic cells.
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Specimen
8E/G:
embryonic day 14.5
(more )
Note: Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
Specimen
8E/G:
(close )
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Genetic Background:
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involves: Swiss
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
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Specimen
8F/H:
embryonic day 14.5; Pax6Sey/ Pax6Sey
(more )
Note: Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
Specimen
8F/H:
(close )
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Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Mash1 (Ascl1); red - pH3 (phospho-histone 3); blue - DNA.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Weak |
Regionally restricted |
8F
,
8H
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Low levels of expression in a minority of cells undergoing mitosis in abventricular locations.
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Analysis Tools