Antibody:	anti-Sox-9 (H-90; sc-20095)
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: dental placode	Absent			No expression at lamina stage (E11.5).

Structure	Level	Pattern	Image	Note
TS20: dental placode	Absent			No expression at early bud stage (E12.5).

Structure	Level	Pattern	Image	Note
TS21: tooth bud	Strong	Regionally restricted	3a	At late bud stage, strong expression in dental epithelium.
TS21: incisor epithelium	Strong	Regionally restricted	3a	At late bud stage, strong expression in dental epithelium.

Structure	Level	Pattern	Image	Note
TS22: cervical loop	Strong	Regionally restricted	3b	Highly expressed in most cells within the dental epithelium including labial and lingual cervical loop.
TS22: primary enamel knot	Absent		3b

Structure	Level	Pattern	Image	Note
TS24: incisor epithelium	Present		3d	Expression in both epithelium and mesenchyme.
TS24: incisor mesenchyme	Present		3d	Expression in both epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: incisor mesenchyme	Present	Regionally restricted	3d'	Expression in mesenchymal cells within the dental pulp, especially mesenchyme adjacent to labial cervical loop.

Structure	Level	Pattern	Image	Note
TS24: cervical loop	Strong	Regionally restricted	3d"	Strong expression in labial cervical loop, including basal epithelium, stellate reticulum and outer enamel epitheium. Expression also in lingual cervical loop area.
TS24: outer enamel epithelium	Strong	Regionally restricted	3d"	Strong expression in labial cervical loop, including basal epithelium, stellate reticulum and outer enamel epitheium. Expression also in lingual cervical loop area.
TS24: stellate reticulum	Strong	Regionally restricted	3d"	Strong expression in labial cervical loop, including basal epithelium, stellate reticulum and outer enamel epitheium. Expression also in lingual cervical loop area.

Structure	Level	Pattern	Image	Note
TS26: incisor epithelium	Present		3e	Authors report similar expression with that at E16.5
TS26: incisor mesenchyme	Present		3e	Authors report similar expression with that at E16.5

Structure	Level	Pattern	Image	Note
TS26: incisor mesenchyme	Present	Regionally restricted	3e'	Expression in mesenchymal cells within the dental pulp, especially mesenchyme adjacent to labial cervical loop.

Structure	Level	Pattern	Image	Note
TS26: cervical loop	Strong	Regionally restricted	3e"	Strong expression in labial cervical loop, including basal epithelium, stellate reticulum and outer enamel epitheium.
TS26: outer enamel epithelium	Strong	Regionally restricted	3e"	Strong expression in labial cervical loop, including basal epithelium, stellate reticulum and outer enamel epitheium.
TS26: stellate reticulum	Strong	Regionally restricted	3e"	Strong expression in labial cervical loop, including basal epithelium, stellate reticulum and outer enamel epitheium.

Structure	Level	Pattern	Image	Note
TS27: incisor epithelium	Present		4a	Expression in both epithelium and mesenchyme.
TS27: incisor mesenchyme	Present		4a	Expression in both epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS27: cervical loop	Strong	Regionally restricted	4a'	Strong expression in labial cervical loop, including basal epitheium, stellate reticulum and outer enamel epithelium. No expression in matured ameloblasts.
TS27: outer enamel epithelium	Strong	Regionally restricted	4a'	Strong expression in labial cervical loop, including basal epitheium, stellate reticulum and outer enamel epithelium. No expression in matured ameloblasts.
TS27: stellate reticulum	Strong	Regionally restricted	4a'	Strong expression in labial cervical loop, including basal epitheium, stellate reticulum and outer enamel epithelium. No expression in matured ameloblasts.

Structure	Level	Pattern	Image	Note
TS27: incisor mesenchyme	Strong	Regionally restricted	4a"	Strong expression in dental mesenchyme, especially in cells adjacent to epithelium. No expression in matured odontoblast.

Structure	Level	Pattern	Image	Note
TS28: incisor epithelium	Present		4b	Expression in both epithelium and mesenchyme.
TS28: incisor mesenchyme	Present		4b	Expression in both epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS28: cervical loop	Strong	Regionally restricted	4b'	Strong expression in labial cervical loop, including basal epitheium, stellate reticulum and outer enamel epithelium. No expression in matured ameloblasts.
TS28: outer enamel epithelium	Strong	Regionally restricted	4b'	Strong expression in labial cervical loop, including basal epitheium, stellate reticulum and outer enamel epithelium. No expression in matured ameloblasts.
TS28: stellate reticulum	Strong	Regionally restricted	4b'	Strong expression in labial cervical loop, including basal epitheium, stellate reticulum and outer enamel epithelium. No expression in matured ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: incisor mesenchyme	Strong	Regionally restricted	4b"	Strong expression in dental mesenchyme, especially in cells adjacent to epithelium. No expression in matured odontoblast.