Probe:

MGP probe2

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present	Regionally restricted	6a/b	At E12.5, expression throughout lung mesenchyme at the epithelial-mesenchymal interface.
TS20: bronchus	Strong		6a/b	Strong expression in developing trachea and bronchi.
TS20: trachea	Strong		6a/b	Strong expression in developing trachea and bronchi.
TS20: connective tissue	Strong	Regionally restricted	6a/b	Strong expression in connective tissue membranes.
TS20: vertebral cartilage condensation	Strong	Regionally restricted	6a/b	Strong expression in chondrocytes of vertebral bodies.
TS20: liver	Absent		6a/b	No expression in cells of liver and spinal ganglia.
TS20: spinal ganglion	Absent		6a/b	No expression in cells of liver and spinal ganglia.

Structure	Level	Pattern	Image	Note
TS22: trachea	Present	Regionally restricted	6c/d	Expression in submucosal layer underlying epithelium of trachea
TS22: bronchus	Strong	Regionally restricted	6c/d	Expression in submucosal layer underlying epithelium of bronchi. Expression most intense in dividing bronchi.
TS22: bronchiole	Present	Regionally restricted	6c/d	Expression in submucosal layer underlying epithelium of larger bronchioles.
TS22: lung	Present	Regionally restricted	6c/d	Expression in at epithelial-mesenchymal interface of pulmonary alveoli. Diffuse expression within alveolar cells.
TS22: vertebral cartilage condensation	Present	Regionally restricted	6c/d	Expression persists in chondrocytes within vertebral bodies.
TS22: pleura	Present		6c/d
TS22: cardiac muscle tissue	Absent		6c/d

Structure	Level	Pattern	Image	Note
TS25: lobar bronchus mesenchyme	Present	Regionally restricted	6e/f
TS25: lung alveolus	Absent		6e/f
TS25: terminal bronchiole	Absent		6e/f

Structure	Level	Pattern	Image	Note
TS25: trachea	Present
TS25: bronchiole	Present	Regionally restricted
TS25: bronchiole epithelium	Absent
TS25: trachea epithelium	Absent

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process mesenchyme	Strong	Regionally restricted	7a/b

Structure	Level	Pattern	Image	Note
TS25: chondrocranium cartilage condensation	Present	Regionally restricted	7c/d	Expression in chondrocytes within the chondrocranium - in the basioccital region of skull.
TS25: chondrocranium bone	Absent		7c/d	No signal above background in calvarial osteoblasts and cells of neuronal tissues.
TS25: brain	Absent		7c/d

Structure	Level	Pattern	Image	Note
TS27: Meckel's cartilage	Present		7e/f
TS27: lower jaw molar dental papilla	Absent		7e/f	No expression in odontoblasts within molar.
TS27: lower jaw molar dental follicle	Absent		7e/f	No expression in alveolar osteoblasts.
TS27: mandible	Present	Regionally restricted	7e/f	Less intense expression in periosteal cells.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted		Authors report the same pattern of expression is observed in limb buds at E12.5.

Structure	Level	Pattern	Image	Note
TS25: forelimb	Present	Regionally restricted		Authors report the same pattern of expression is observe in forelimb and hindlimb at E17.5.
TS25: hindlimb	Present	Regionally restricted		Authors report the same pattern of expression is observe in forelimb and hindlimb at E17.5.

Structure	Level	Pattern	Image	Note
TS25: renal medulla	Present		7g/h
TS25: renal cortex	Absent		7g/h

Structure	Level	Pattern	Image	Note
TS26: uterine horn muscular layer	Present	Regionally restricted	7i/j	Expression in smooth muscle cells in uterine wall.

Structure	Level	Pattern	Image	Note
TS27: ulna	Strong	Regionally restricted	8b/c	High level of expression in resting and proliferating chondrocytes. No expression in in zone of ossification.
TS27: hypertrophic cartilage zone	Strong	Regionally restricted	8b/c	High level of expression in cells within the zone of late hypertrophic chondrocytes.No expression in zone of early hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS27: ulna	Present	Regionally restricted	9c/d	Expression in proliferative chondrocytes. No expression in osteoblasts in the zone of ossification.
TS27: hypertrophic cartilage zone	Present	Regionally restricted	9c/d	Expression in late hypertrophic chondrocytes. No expression in early hypertrophic .