Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142157
McBratney-Owen B, et al., Development and tissue origins of the mammalian cranial base. Dev Biol. 2008 Oct 1;322(1):121-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6159293
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Probe:
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Col2 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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All embryos were first staged using criteria established by Theiler (1989) and Miyake et al. (1996) so that chondrocranial development could be observed relative to developmental stage rather than conception date. After being designated a specific stage (T18 to T24), embryos were labeled according to the general corresponding conception age (E11 to E16).
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Results
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Specimen
E11 not shown:
embryonic day 11.0
(more  )
Specimen
E11 not shown:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: chordamesoderm |
Present |
Regionally restricted |
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Expression in parachordal cartilage at E11.
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Specimen
3A:
embryonic day 12.0
(more )
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B:
embryonic day 13.0
(more )
Specimen
3B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3C:
embryonic day 14.0
(more )
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
Present |
Regionally restricted |
3C
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Expression where the acrochordal cartilage will develop, which creates the rostral border of the basicranial fenestra.
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Specimen
3D:
embryonic day 15.0
(more )
Specimen
3D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: chondrocranium |
Strong |
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3D
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Strongest expression in the chondrocranium at E15.
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Specimen
3E:
embryonic day 16.0
(more )
Specimen
3E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: chondrocranium bone |
Weak |
Regionally restricted |
3E
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Expression decreases in the parachordal and hypophyseal anlagen where endochondral ossification is beginning.
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Analysis Tools