Mt2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240319 Stauber M, et al., Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse. Dev Biol. 2017 Mar 15;423(2):170-188
Assay type:	RT-PCR
MGI Accession ID:	MGI:6157697
Gene symbol:	Mt2
Gene name:	metallothionein 2

Probe:	Mt2 pair_47
Assay notes:	Quantitative RT-PCR. Measuring relative expression of wild type and mutant. Hprt served as reference. Supplemental Table 2 - Sheet 1.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	102 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Sample 1 WT	E18.5	TS26: lung	Present	0.05 µg; total RNA	involves: 129X1/SvJ * CD-1		Not Specified	qPCR run D_1
Sample 1 Foxj1-/-	E18.5	TS26: lung	Present (a)	0.05 µg; total RNA	involves: 129X1/SvJ * CD-1	Foxj1^tm1Slb/Foxj1^tm1Slb	Not Specified	qPCR run D_1
Sample 1 WT	E18.5	TS26: lung	Present	0.05 µg; total RNA	NOD.NON-H2^nb1		Not Specified	qPCR run M_1
Sample 1 Foxj1-/-	E18.5	TS26: lung	Present (b)	0.05 µg; total RNA	involves: 129X1/SvJ * CD-1	Foxj1^tm1Slb/Foxj1^tm1Slb	Not Specified	qPCR run M_1
Sample 2 WT	E18.5	TS26: lung	Present	0.04 µg; total RNA	involves: 129X1/SvJ * CD-1		Not Specified	qPCR run K_2,L_2
Sample 2 Foxj1-/-	E18.5	TS26: lung	Present (c)	0.04 µg; total RNA	involves: 129X1/SvJ * CD-1	Foxj1^tm1Slb/Foxj1^tm1Slb	Not Specified	qPCR run K_2,L_2

Notes:
(a) Expression was down-regulated in the mutant by 0-2-fold.
(b) Expression was not down-regulated in the mutant (<0-fold).
(c) Expression was down-regulated in the mutant by greater than 2-fold.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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