Gene Expression Data
Assay Details
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Assay
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Reference:
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J:255527
Broix L, et al., Ciliogenesis and cell cycle alterations contribute to KIF2A-related malformations of cortical development. Hum Mol Genet. 2018 Jan 15;27(2):224-238
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6156270
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Gene symbol:
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Kif2a
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Gene name:
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kinesin family member 2A
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Antibody:
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anti-Kif2a
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
S1B left column:
embryonic day 16.0
(more  )
Note: Double labeled: green - Kif2a; red - Pax6 (assay MGI:6156272)
Specimen
S1B left column:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: green - Kif2a; red - Pax6 (assay MGI:6156272)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex ventricular layer |
Present |
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S1B left Kif2a
,
S1B left Merge (hi pwr)
,
S1B left Merge
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As demonostrated by the double staining, expression is seen in proliferating cells/radial glia.
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Specimen
S1B right column:
embryonic day 16.0
(more )
Note: Double labeled: green - Kif2a; red - Tubb3 (assay MGI:6156273)
Specimen
S1B right column:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: green - Kif2a; red - Tubb3 (assay MGI:6156273)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex intermediate zone |
Present |
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S1B right Kif2a
,
S1B right Merge (hi pwr)
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S1B right Merge
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As demonstrated by the double staining, expression is seen in postmitotic neurons.
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| TS24: cortical plate |
Present |
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S1B right Kif2a
,
S1B right Merge (hi pwr)
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S1B right Merge
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As demonstrated by the double staining, expression is seen in postmitotic neurons.
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