Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96531
Huber K, et al., The role of Phox2B in chromaffin cell development. Dev Biol. 2005 Mar 15;279(2):501-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6155737
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Gene symbol:
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Nefl
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Gene name:
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neurofilament, light polypeptide
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Probe:
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Nefl probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
6A:
embryonic day 14.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: sympathetic ganglion |
Present |
Regionally restricted |
6A
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Expression in suprarenal ganglion.
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| TS22: adrenal gland |
Trace |
Regionally restricted |
6A
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Expression in very few cells, presumably neurons within the adrenal gland.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: adrenal medulla |
Present |
Widespread |
6B
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Expression in many adrenal medullary cells.
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Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: adrenal gland |
Absent |
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6C
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No expression in adrenal sympathoadrenal (SA) cells.
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Specimen
7A:
embryonic day 12.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: migrating neural crest |
Present |
Regionally restricted |
7A
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Expression in developing adrenal chromaffin cell progenitors when sympathoadrenal (SA) cells have just begun to colonize the adrenal gland.
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Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
