Antibody:	Anti-PKD3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1C PKD3

Structure	Level	Pattern	Image	Note
TS16: primitive ventricle	Strong		2C

Structure	Level	Pattern	Image	Note
TS16: heart atrium	Strong		2D
TS16: nasal process	Present		2D

Structure	Level	Pattern	Image	Note
TS16: nasal process	Present		2E
TS16: primitive ventricle	Strong		2E

Structure	Level	Pattern	Image	Note
TS16: dermomyotome	Present		2F
TS16: blood	Absent		2F	Erythrocytes in the atrium and dorsal aorta were negative.

Structure	Level	Pattern	Image	Note
TS16: dermomyotome	Present		2G
TS16: notochord	Present		2G

Structure	Level	Pattern	Image	Note
TS16: nasal process	Present		2H
TS16: notochord	Present		2H

Structure	Level	Pattern	Image	Note
TS16: dermomyotome	Present		2I
TS16: forelimb bud	Present		2I
TS16: blood	Absent		2I	Erythrocytes in the atrium and dorsal aorta were negative.
TS16: heart atrium	Strong		2I

Structure	Level	Pattern	Image	Note
TS16: future forebrain	Absent
TS16: future midbrain	Absent

Structure	Level	Pattern	Image	Note
TS19: head	Present	Regionally restricted	3C	Expression was detected in the head near the developing base of the skull and nasal process.
TS19: cardiac muscle tissue	Present		3C
TS19: dorsal aorta	Present		3C
TS19: main bronchus epithelium	Present		3C

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		3D
TS19: main bronchus epithelium	Present		3D

Structure	Level	Pattern	Image	Note
TS19: head	Present	Regionally restricted	3E	Expression was detected in the head near the developing base of the skull and nasal process.

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		3F

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		3G

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present		3H
TS19: notochord	Present		3H

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present		3I

Structure	Level	Pattern	Image	Note
TS19: main bronchus epithelium	Strong		3J

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	4C	Expression was detected in the outer layer.
TS20: heart atrium	Present		4C
TS20: heart ventricle	Present		4C

Structure	Level	Pattern	Image	Note
TS20: temporal bone	Present		4D

Structure	Level	Pattern	Image	Note
TS20: nasal mesenchyme	Present	Regionally restricted	4E	Expression was detected in the cartilage primordium of nasal bones.

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		4F
TS20: vertebral cartilage condensation	Present		4F
TS20: esophagus	Strong	Regionally restricted	4F	Strong expression was noted in the esophagus membrane.

Structure	Level	Pattern	Image	Note
TS20: esophagus	Strong	Regionally restricted	4F'	Strong expression was noted in the esophagus membrane.

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		4F''

Structure	Level	Pattern	Image	Note
TS20: heart atrium	Present		4G
TS20: heart ventricle	Present		4G
TS20: atrioventricular endocardial cushion	Absent		4G	Expression was absent from the atrio-ventricular bulbar cushion tissue.

Structure	Level	Pattern	Image	Note
TS20: bronchus epithelium	Strong	Regionally restricted	4H	Strong expression was detected in epithelial cells of segmental bronchii.

Structure	Level	Pattern	Image	Note
TS20: urogenital ridge	Present		4I

Structure	Level	Pattern	Image	Note
TS20: temporal bone	Present		4J

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	4K	Expression was detected in the outer layer.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4L	Expression was detected in the mucous membrane.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Absent		5C
TS21: liver	Absent		5C
TS21: spinal cord marginal layer	Present		5C
TS21: bronchus epithelium	Present	Regionally restricted	5C	The epithelial layer of the segmental but not the main bronchi was positive.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Absent		5D
TS21: liver	Absent		5D
TS21: 4th ventricle choroid plexus	Present		5D
TS21: spinal cord marginal layer	Present		5D
TS21: stomach	Present	Regionally restricted	5D	Prominent expression was detected in the middle layer (submucosa) of the stomach, but not the inner layer (mucosa).

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		5E	Expression was detected in cardiomyocytes.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Strong	Regionally restricted	5F	Expression was detected in skeletal muscle cells of the neck region.

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Strong		5G

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Strong		5G'

Structure	Level	Pattern	Image	Note
TS21: main bronchus epithelium	Absent		5H
TS21: bronchus epithelium	Present	Regionally restricted	5H	The epithelial layer of the segmental but not the main bronchi was positive.

Structure	Level	Pattern	Image	Note
TS21: main bronchus epithelium	Absent		5H'
TS21: bronchus epithelium	Present	Regionally restricted	5H'	The epithelial layer of the segmental but not the main bronchi was positive.

Structure	Level	Pattern	Image	Note
TS21: stomach	Present	Regionally restricted	5I	Prominent expression was detected in the middle layer (submucosa) of the stomach, but not the inner layer (mucosa).

Structure	Level	Pattern	Image	Note
TS21: stomach	Present	Regionally restricted	5I'	Prominent expression was detected in the middle layer (submucosa) of the stomach, but not the inner layer (mucosa).

Structure	Level	Pattern	Image	Note
TS21: spinal cord marginal layer	Present		5J

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		5K

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		5K'

Structure	Level	Pattern	Image	Note
TS21: cranium bone primordium	Present	Regionally restricted	5L	Expression was detected in the cartilage primordium of turbinate bones.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	5M	Intense expression was detected in osteoblasts.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	5M'	Intense expression was detected in osteoblasts.
TS21: perichondrium	Present		5M'

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Present		5N

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	6D	Expression in the brain was ubiquitous with the exception of the neocortex, which was negative.
TS22: neocortex	Absent		6D

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	6E	Expression in the brain was ubiquitous with the exception of the neocortex, which was negative.
TS22: neocortex	Absent		6E
TS22: 4th ventricle choroid plexus	Present		6E
TS22: lateral ventricle choroid plexus	Present		6E

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	6F	Expression in the brain was ubiquitous with the exception of the neocortex, which was negative.
TS22: neocortex	Absent		6F

Structure	Level	Pattern	Image	Note
TS22: vertebra	Present	Regionally restricted	6G	Expression was even more prominent in E14.5 osteoblasts (compared to E13.5).

Structure	Level	Pattern	Image	Note
TS22: nucleus pulposus	Present		6H

Structure	Level	Pattern	Image	Note
TS22: nucleus pulposus	Present		6H'

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Strong	Regionally restricted	6I	Skeletal muscle cells of the diaphragm showed a strong expression.

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Strong	Regionally restricted	6I'	Skeletal muscle cells of the diaphragm showed a strong expression.

Structure	Level	Pattern	Image	Note
TS22: renal capsule	Strong		6J

Structure	Level	Pattern	Image	Note
TS22: renal capsule	Strong		6J'

Structure	Level	Pattern	Image	Note
TS22: bronchiole	Strong	Regionally restricted	6K	Strong expression was detected in the terminal bronchioles of the lung.

Structure	Level	Pattern	Image	Note
TS22: liver	Strong		6L

Structure	Level	Pattern	Image	Note
TS22: duodenum	Strong	Regionally restricted	6M	Strong expression was detected in the middle layer of the duodenum.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	6N	Distinct nerve tracts were intensely stained.

Structure	Level	Pattern	Image	Note
TS22: lateral ventricle choroid plexus	Present		6O

Structure	Level	Pattern	Image	Note
TS22: retina	Weak	Regionally restricted	6P	Weak staining was noted in the inner layer of the retina.
TS22: extrinsic ocular muscle	Strong		6P

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Strong		6Q

Structure	Level	Pattern	Image	Note
TS22: vibrissa root sheath	Present		6R

Structure	Level	Pattern	Image	Note
TS22: vibrissa root sheath	Present		6R'

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	6S	Expression was detected in the suprabasal layer of the epidermis.
TS22: connective tissue	Present	Regionally restricted	6S	Expression was detected in the connective tissue underlying the epidermis.

Structure	Level	Pattern	Image	Note
TS22: oral region	Present	Regionally restricted	6T	Expression was detected in ectoderm-derived epidermal layers but was absent from endoderm.
TS22: epidermis	Present		6T

Structure	Level	Pattern	Image	Note
TS22: upper jaw molar	Present		6U

Structure	Level	Pattern	Image	Note
TS24: embryo	Present	Widespread	7C
TS24: liver	Present		7C

Structure	Level	Pattern	Image	Note
TS24: embryo	Present	Widespread	7D
TS24: liver	Present		7D

Structure	Level	Pattern	Image	Note
TS24: pons	Strong	Regionally restricted	7E	Strong expression was detected in the nerve tracts in the pons.

Structure	Level	Pattern	Image	Note
TS24: 4th ventricle choroid plexus	Present		7F

Structure	Level	Pattern	Image	Note
TS24: lateral ventricle choroid plexus	Present		7G

Structure	Level	Pattern	Image	Note
TS24: vibrissa root sheath	Present		7H

Structure	Level	Pattern	Image	Note
TS24: duodenum submucosa	Present		7I
TS24: duodenum mucosa	Absent		7I
TS24: duodenum muscularis	Absent		7I

Structure	Level	Pattern	Image	Note
TS24: stomach wall	Weak	Regionally restricted	7J	Weak expression was noted in the submucosa of the stomach, however, smooth muscle cells in the mucularis layer of the stomach were negative.

Structure	Level	Pattern	Image	Note
TS24: stomach wall	Weak	Regionally restricted	7J'	Weak expression was noted in the submucosa of the stomach, however, smooth muscle cells in the mucularis layer of the stomach were negative.

Structure	Level	Pattern	Image	Note
TS24: renal capsule	Strong		7K

Structure	Level	Pattern	Image	Note
TS24: bronchus	Strong	Regionally restricted	7L	Strong expression was evident in the terminal bronchi of the lung.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong	Regionally restricted	7M	Intensive staining was noted in the suprabasal layer of the epidermis of the lower lip.

Structure	Level	Pattern	Image	Note
TS24: intercostal muscle	Strong		7N

Structure	Level	Pattern	Image	Note
TS24: tongue intrinsic muscle transverse component	Strong		7O

Structure	Level	Pattern	Image	Note
TS24: diaphragm	Present	Regionally restricted	7P	The distribution of staining in skeletal muscle cells was polarized.

Structure	Level	Pattern	Image	Note
TS24: neck muscle	Present	Regionally restricted	7Q	The distribution of staining in skeletal muscle cells was polarized.

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Present		7R

Structure	Level	Pattern	Image	Note
TS24: smooth muscle tissue	Absent
TS24: thymus	Absent