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Gene Expression Data
Assay Details
Assay
Reference: J:254721 Zhou Z, et al., Temporally Distinct Six2-Positive Second Heart Field Progenitors Regulate Mammalian Heart Development and Disease. Cell Rep. 2017 Jan 24;18(4):1019-1032
Assay type: RNA in situ
MGI Accession ID: MGI:6154693
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Probe: Six2 probe5
Probe preparation: Antisense RNA
Results
Specimen 1A: embryonic day 6.0-6.5 (more )
Structure Level Pattern Image Note
TS8: embryo Absent 1A Other experiments suggest expression is present but levels are too low to be detected by in situ hybridization at this age.
Specimen 1B: embryonic day 7.0-7.5 (more )
Note: cardiac cresent stage embryo
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 1B Expression is present in two bilateral domains.
Specimen 1C: embryonic day 8.0 (more )
Note: 2 somite stage embryo
Structure Level Pattern Image Note
TS12: head mesenchyme Strong Regionally restricted 1C Expression is seen flanking, but not in, the developing heart tube.
TS12: heart Absent 1C
Specimen 1D: embryonic day 8.25 (more )
Note: 5 somite stage embryo
Structure Level Pattern Image Note
TS12: head mesenchyme Strong Regionally restricted 1D Expression is seen flanking, but not in, the developing heart tube.
TS12: heart Absent 1D
Specimen 1E: embryonic day 8.75 (more )
Note: 9 somite stage embryo
Structure Level Pattern Image Note
TS13: head mesenchyme Strong Regionally restricted 1E Expression is seen flanking, but not in, the developing heart tube.
TS13: heart Absent 1E
Specimen 1F: embryonic day 9.25 (more )
Note: 14 somite stage embryo
Structure Level Pattern Image Note
TS14: 1st branchial arch Present 1F
TS14: 2nd branchial arch Present 1F
TS14: 3rd branchial arch Present 1F
TS14: fronto-nasal process Present 1F
TS14: head mesenchyme Present 1F
Specimen 1G - 1: embryonic day 8.75 (more )
Note: 9 somite stage embryo
Structure Level Pattern Image Note
TS13: head mesenchyme Present 1G 1st
Specimen 1G - 2: embryonic day 8.75 (more )
Note: 9 somite stage embryo
Structure Level Pattern Image Note
TS13: embryo Present 1G 2nd
Specimen 1G - 3: embryonic day 8.75 (more )
Note: 9 somite stage embryo
Structure Level Pattern Image Note
TS13: branchial groove ectoderm Present 1G 3rd
TS13: branchial pouch endoderm Present 1G 3rd
Specimen 1G - 4: embryonic day 8.75 (more )
Note: 9 somite stage embryo
Structure Level Pattern Image Note
TS13: secondary heart field Present Non-Uniform 1G 4th Expression is seen in the splanchnic mesoderm of the dorsal pericardial wall, which belongs to the secondary heart field. Expression is stronger on the left versus the right side.
TS13: dorsal mesocardium Absent 1G 4th
Specimen 1H - 1: embryonic day 9.25 (more )
Note: 14 somite stage embryo
Structure Level Pattern Image Note
TS14: secondary heart field Absent 1H 1st Expression is absent from the rostral half and the most caudal one-quarter.
Specimen 1H - 2: embryonic day 9.25 (more )
Note: 14 somite stage embryo
Structure Level Pattern Image Note
TS14: secondary heart field Present 1H 2nd
Specimen 1H - 3: embryonic day 9.25 (more )
Note: 14 somite stage embryo
Structure Level Pattern Image Note
TS14: secondary heart field Present 1H 3rd
Specimen 1H - 4: embryonic day 9.25 (more )
Note: 14 somite stage embryo
Structure Level Pattern Image Note
TS14: secondary heart field Present 1H 4th
TS14: embryo Present Regionally restricted 1H 4th Expression is seen in the trachesophageal complex.
Specimen 4A: embryonic day 7.0-7.5; Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Structure Level Pattern Image Note
TS10: embryo Strong Regionally restricted 4A Expression is present in two bilateral domains.
Specimen 4B: embryonic day 7.0-7.5; Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt (more )
Structure Level Pattern Image Note
TS10: embryo Weak Regionally restricted 4B Expression is present in two bilateral domains; expression levels are reduced compared to those seen in wild type.
Specimen 4C: embryonic day 9.0; Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Structure Level Pattern Image Note
TS14: embryo Present Regionally restricted 4C Expression is localized bilaterally along the mediolateral axis and is absent from midline tissues.
Specimen 4D: embryonic day 9.0; Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt (more )
Structure Level Pattern Image Note
TS14: embryo Present Regionally restricted 4D Expression is localized bilaterally along the mediolateral axis and is absent from midline tissues. In the mutant expression is reduced in the rostral and caudal expression subdomains compared to what is seen in wild type.
Specimen 4E: embryonic day 9.0; Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Structure Level Pattern Image Note
TS14: embryo Present 4E
TS14: aortic sac Absent 4E
Specimen 4F: embryonic day 9.0; Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Structure Level Pattern Image Note
TS14: embryo Present 4F
TS14: aortic sac Absent 4F
Specimen 4G: embryonic day 9.0; Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt (more )
Structure Level Pattern Image Note
TS14: secondary heart field Present 4G
TS14: dorsal mesocardium Absent 4G
Specimen 4H: embryonic day 9.0; Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt (more )
Structure Level Pattern Image Note
TS14: secondary heart field Present 4H The signal is reduced in the mutant, particularly on the caudal left side.
TS14: dorsal mesocardium Absent 4H
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory