Gene Expression Data
Assay Details
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Assay
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Reference:
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J:223033
Tomas-Roca L, et al., De novo mutations in PLXND1 and REV3L cause Mobius syndrome. Nat Commun. 2015;6:7199
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6154630
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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clone 39.4D5
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2d wt:
embryonic day 16.5
(more  )
Specimen
2d wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindbrain |
Present |
Regionally restricted |
2d wt
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Expression in facial motor nucleus (FBM) motoneurons. Authors report all FBM mononeurons reached rhombomere 6 (r6) in wild type mice
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Specimen
2d +/-:
embryonic day 16.5; Plxnd1tm1Joe/Plxnd1 +
(more )
Specimen
2d +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Plxnd1tm1Joe/Plxnd1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindbrain |
Present |
Regionally restricted |
2d +/-
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Expression in migrating motoneurons outside the facial nucleus, along the migratory path of the facial nerve. Authors report a subpopulation of motoneurons still located in rhombomres 4 and 5 (r4 and r5) at E16.5
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Specimen
2d -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Plxnd1tm1Joe/Plxnd1tm1Joe
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindbrain |
Present |
Regionally restricted |
2d -/-
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Expression in migrating motoneurons outside the facial nucleus, along the migratory path of the facial nerve. Authors report a subpopulation of motoneurons still located in rhombomres 4 and 5 (r4 and r5) at E16.5
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Analysis Tools