Antibody:	Lumican antibody
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		1D

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		1D'
TS17: outflow tract	Present	Regionally restricted	1D'	Expression is in the non-cellularized regions.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		1D''
TS17: atrioventricular endocardial cushion	Present	Regionally restricted	1D''	Expression is in the non-cellularized regions.

Structure	Level	Pattern	Image	Note
TS22: pulmonary artery	Present	Regionally restricted	2S	Expression is in the adventitial layer.
TS22: great vessel of heart	Present	Regionally restricted	2S	Expression is in the adventitial layer of the wall of the aorta.

Structure	Level	Pattern	Image	Note
TS22: pulmonary artery	Present	Regionally restricted	2T	Expression is in the adventitial layer.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present	Regionally restricted	2U	Expression is adjacent to the valve mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present	Regionally restricted	2V	Expression is adjacent to the valve mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: great vessel of heart	Present	Regionally restricted	2W	Expression is in the adventitial layer of the wall of the aorta. This is not seen in this specimen which has been fixed using Amsterdam fixative.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Present		2X

Structure	Level	Pattern	Image	Note
TS27: pulmonary artery	Very strong	Regionally restricted	3P , 3P'	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
TS27: pulmonary valve	Very strong	Regionally restricted	3P , 3P'	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.

Structure	Level	Pattern	Image	Note
TS27: pulmonary artery	Very strong	Regionally restricted	3Q	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
TS27: pulmonary valve	Very strong	Regionally restricted	3Q	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.

Structure	Level	Pattern	Image	Note
TS27: aorta tunica adventitia	Very strong		3R
TS27: left ventricle cardiac muscle	Weak	Regionally restricted	3R	Expression is at the myocardial attachment regions of the cusps.

Structure	Level	Pattern	Image	Note
TS27: mitral valve	Present	Regionally restricted	3S	Expression is the leaflets but is absent in the region where the leaflets anchor into the myocardium.

Structure	Level	Pattern	Image	Note
TS27: pericardium	Present	Regionally restricted	3T	Expression is subjacent to the epicardial epithelial cell layer.

Structure	Level	Pattern	Image	Note
TS28: pulmonary valve	Trace	Regionally restricted	4M	Expression is in regions where the cusp hinges anchor to surrounding tissues and is absent in the cusps.
TS28: pulmonary valve anulus	Present		4M
TS28: aortic valve	Present	Regionally restricted	4M	Expression is in the distal cusps and is absent the valve hinge area (shown).

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Present	Regionally restricted	4N , 4N'	Expression is in the distal cusps and is absent the valve hinge area (shown).
TS28: aorta tunica media	Present	Regionally restricted	4N'' , 4N	Expression is in the outer aspect.

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Present	Regionally restricted	4O	Expression is in the distal cusps.

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Present	Regionally restricted	4P	Expression is concentrated in the tip of the anterior leaflet is virtually absent in fibrous attachment of the posterior leaflet.
TS28: chordae tendinae	Present		4P

Structure	Level	Pattern	Image	Note
TS28: semilunar valve	Present		5J
TS28: pulmonary artery	Strong	Regionally restricted	5J	Expression is in the adventitia.

Structure	Level	Pattern	Image	Note
TS28: semilunar valve	Present		5K , 5K'
TS28: aorta	Strong		5K , 5K'
TS28: pulmonary artery	Strong	Regionally restricted	5K , 5K'	Expression is in the adventitia.

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Strong		5L , 5L'