Antibody:	Fibromodulin antibody (LF150)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		1C

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		1C'
TS17: atrioventricular endocardial cushion	Trace	Regionally restricted	1C'	Expression is in the non-cellularized regions.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		1C''
TS17: atrioventricular endocardial cushion	Trace	Regionally restricted	1C''	Expression is in the non-cellularized regions.

Structure	Level	Pattern	Image	Note
TS22: great vessel of heart	Present	Regionally restricted	2M	Expression is in the adventitial layer of the wall of the aorta.
TS22: pulmonary artery	Present	Regionally restricted	2M	Expression is in the adventitial layer.

Structure	Level	Pattern	Image	Note
TS22: pulmonary artery	Present	Regionally restricted	2N	Expression is in the adventitial layer.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present	Regionally restricted	2O	Expression is adjacent to the valve mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present	Regionally restricted	2P	Expression is adjacent to the valve mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: great vessel of heart	Present	Regionally restricted	2Q	Expression is in the adventitial layer of the wall of the aorta. This is not seen in this specimen which has been fixed using Amsterdam fixative.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Absent		2R

Structure	Level	Pattern	Image	Note
TS27: pulmonary valve	Very strong	Regionally restricted	3K , 3K'	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.

Structure	Level	Pattern	Image	Note
TS27: pulmonary valve	Very strong	Regionally restricted	3L	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.

Structure	Level	Pattern	Image	Note
TS27: left ventricle cardiac muscle	Present	Regionally restricted	3M	Expression is at the myocardial attachment regions of the cusps.
TS27: aorta	Absent		3M
TS27: pulmonary artery	Absent		3M

Structure	Level	Pattern	Image	Note
TS27: mitral valve	Present	Regionally restricted	3N	Expression is in areas where the leaflets anchor into the myocardium.

Structure	Level	Pattern	Image	Note
TS27: pericardium	Absent		3O

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Very strong	Regionally restricted	4I	Expression is in regions where the cusp hinges anchor to surrounding tissues.
TS28: pulmonary valve	Very strong	Regionally restricted	4I	Expression is in regions where the cusp hinges anchor to surrounding tissues and is absent in the cusps.
TS28: pulmonary valve anulus	Present		4I

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Very strong	Regionally restricted	4J , 4J'	Expression is in regions where the cusp hinges anchor to surrounding tissues and in the cusp hinge.
TS28: aorta wall	Absent		4J , 4J''

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Present	Regionally restricted	4K	Expression is in regions where the cusp hinges anchor to surrounding tissues and in the cusp hinge and is absent in the distal cusps (shown).

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Present	Regionally restricted	4L	Expression is in fibrous attachment of the posterior leaflet.
TS28: chordae tendinae	Present		4L

Structure	Level	Pattern	Image	Note
TS28: semilunar valve	Absent		5G

Structure	Level	Pattern	Image	Note
TS28: aorta	Present	Regionally restricted	5H , 5H'	Expression is restricted to regions where the aortic hinge anchored into the surrounding arterial tissue and is absent in regions of the arterial walls that were not associated with cusps.
TS28: pulmonary artery	Present	Regionally restricted	5H , 5H'	Expression is restricted to regions where the aortic hinge anchored into the surrounding arterial tissue and is absent in regions of the arterial walls that were not associated with cusps.
TS28: semilunar valve	Absent		5H , 5H'

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Strong		5I , 5I'