Antibody:	Biglycan antibody (LF-159)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Weak	Punctate	1A	Expression is in the undifferentiated myocardium.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Punctate	1A'	Expression is in the undifferentiated myocardium.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Punctate	1A''	Expression is in the undifferentiated myocardium.

Structure	Level	Pattern	Image	Note
TS22: pulmonary artery	Present	Regionally restricted	2A	Expression is in the wall.
TS22: great vessel of heart	Present	Regionally restricted	2A	Expression is in the wall of the aorta.

Structure	Level	Pattern	Image	Note
TS22: pulmonary artery	Present	Regionally restricted	2B	Expression is in the medial layer.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present	Regionally restricted	2C	Expression is adjacent to the valve mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: great vessel of heart	Present	Regionally restricted	2D	Expression is in the medial layer of the aorta.

Structure	Level	Pattern	Image	Note
TS22: great vessel of heart	Present	Regionally restricted	2E	Expression is in the medial layer of the aorta.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Absent		2F

Structure	Level	Pattern	Image	Note
TS27: pulmonary artery	Very strong	Regionally restricted	3A' , 3A	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
TS27: pulmonary valve	Very strong	Regionally restricted	3A' , 3A	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.

Structure	Level	Pattern	Image	Note
TS27: pulmonary artery	Very strong	Regionally restricted	3B	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
TS27: pulmonary valve	Very strong	Regionally restricted	3B	Expression is at the site where the pulmonary valve cusps attach to the arterial wall.

Structure	Level	Pattern	Image	Note
TS27: left ventricle cardiac muscle	Present	Regionally restricted	3C	Expression is at the myocardial attachment regions of the cusps.
TS27: aorta tunica media	Present		3C

Structure	Level	Pattern	Image	Note
TS27: mitral valve	Strong	Regionally restricted	3D	Expression is in areas where the leaflets anchor into the myocardium.

Structure	Level	Pattern	Image	Note
TS27: pericardium	Weak	Regionally restricted	3E	Expression is in the epicardium.

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Very strong	Regionally restricted	4A	Expression is in regions where the cusp hinges anchor to surrounding tissues.
TS28: pulmonary valve	Very strong	Regionally restricted	4A	Expression is in regions where the cusp hinges anchor to surrounding tissues and is absent in the cusps.
TS28: pulmonary valve anulus	Present		4A

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Very strong	Regionally restricted	4B' , 4B	Expression is in regions where the cusp hinges anchor to surrounding tissues and in the cusp hinge.
TS28: aorta tunica adventitia	Very strong		4B'' , 4B
TS28: aorta tunica media	Present		4B , 4B''

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Present	Regionally restricted	4C	Expression is in the distal cusps.

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Present	Regionally restricted	4D	Expression is concentrated in the tip of the anterior leaflet and is strong in fibrous attachment of the posterior leaflet.
TS28: chordae tendinae	Present		4D

Structure	Level	Pattern	Image	Note
TS28: semilunar valve	Present		5A
TS28: pulmonary artery	Present	Regionally restricted	5A	Expression is in the medial layer.

Structure	Level	Pattern	Image	Note
TS28: semilunar valve	Present		5B' , 5B
TS28: aorta	Strong		5B , 5B'
TS28: pulmonary artery	Strong	Regionally restricted	5B , 5B'	Expression is in the medial layer.

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Strong		5C' , 5C